Protocol for isolating specific C. elegans neuron types for bulk and single-cell RNA sequencing

Autor: Seth R. Taylor, Rebecca D. McWhirter, Brittany K. Matlock, David K. Flaherty, David M. Miller, III

Publikováno v: STAR Protocols, Vol 5, Iss 4, Pp 103439- (2024)

Summary: The C. elegans nervous system is compact and well described, ideal for identifying genetic programs that drive neuron-specific development, function, and connectivity. Here, we present a protocol for isolating specific neuron types for gene

Externí odkaz: https://doaj.org/article/fb4ba01f344d487380337f2a50b6a9b5

In silico analysis of the transcriptional regulatory logic of neuronal identity specification throughout the C. elegans nervous system

Autor: Lori Glenwinkel, Seth R Taylor, Kasper Langebeck-Jensen, Laura Pereira, Molly B Reilly, Manasa Basavaraju, Ibnul Rafi, Eviatar Yemini, Roger Pocock, Nenad Sestan, Marc Hammarlund, David M Miller III, Oliver Hobert

Publikováno v: eLife, Vol 10 (2021)

The generation of the enormous diversity of neuronal cell types in a differentiating nervous system entails the activation of neuron type-specific gene batteries. To examine the regulatory logic that controls the expression of neuron type-specific ge

Externí odkaz: https://doaj.org/article/af7e725ca0754a56a5647a1273beb41c

The neuropeptidergic connectome ofC. elegans

Autor: Lidia Ripoll-Sánchez, Jan Watteyne, HaoSheng Sun, Robert Fernandez, Seth R Taylor, Alexis Weinreb, Mark Hammarlund, David M Miller, Oliver Hobert, Isabel Beets, Petra E Vértes, William R Schafer

SummaryEfforts are currently ongoing to map synaptic wiring diagrams or connectomes in order to understand the neural basis of brain function. However, chemical synapses represent only one type of functionally important neuronal connection; in partic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::36bdcc3c8c907faa314f04386a844624
https://doi.org/10.1101/2022.10.30.514396

In silico analysis of the transcriptional regulatory logic of neuronal identity specification throughout the C. elegans nervous system

Autor: Marc Hammarlund, Laura Pereira, Molly B. Reilly, Nenad Sestan, Eviatar Yemini, Seth R. Taylor, Manasa Basavaraju, Lori Glenwinkel, Roger Pocock, Oliver Hobert, Ibnul Rafi, Kasper Langebeck-Jensen, David M. Miller

Publikováno v: eLife
Glenwinkel, L, Taylor, S R, Langebeck-Jensen, K, Pereira, L, Reilly, M B, Basavaraju, M, Rafi, I, Yemini, E, Pocock, R, Sestan, N, Hammarlund, M, Miller, D M & Hobert, O 2021, ' In silico analysis of the transcriptional regulatory logic of neuronal identity specification throughout the c. Elegans nervous system ', eLife, vol. 10, e64906, pp. 1-29 . https://doi.org/10.7554/eLife.64906
eLife, Vol 10 (2021)

The generation of the enormous diversity of neuronal cell types in a differentiating nervous system entails the activation of neuron type-specific gene batteries. To examine the regulatory logic that controls the expression of neuron type-specific ge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66ada1d3f86da46d0b2b940e043a9073
http://europepmc.org/articles/PMC8225391

Self-administration of ethanol, cocaine, or nicotine does not decrease the soma size of ventral tegmental area dopamine neurons.

Autor: Michelle S Mazei-Robison, Raghu Appasani, Scott Edwards, Sunmee Wee, Seth R Taylor, Marina R Picciotto, George F Koob, Eric J Nestler

Publikováno v: PLoS ONE, Vol 9, Iss 4, p e95962 (2014)

Our previous observations show that chronic opiate administration, including self-administration, decrease the soma size of dopamine (DA) neurons in the ventral tegmental area (VTA) of rodents and humans, a morphological change correlated with increa

Externí odkaz: https://doaj.org/article/41aba834e69845609d44eb44f00768da