Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Seth E. Lyon"'
Publikováno v:
Frontiers in Microbiology, Vol 15 (2024)
An improved approach was developed for the genetic manipulation of the Gram-positive extremophile Halalkalibacterium halodurans (formerly called Bacillus halodurans). We describe an allelic replacement method originally developed for Staphylococcus a
Externí odkaz:
https://doaj.org/article/af2a6a142e314830a952729377d8da1c
Publikováno v:
Journal of Biological Chemistry. 298:102674
Ornate, large, extremophilic (OLE) RNAs represent a class of noncoding RNAs prevalent in Gram-positive, extremophilic/anaerobic bacterial species. OLE RNAs (∼600 nt), whose precise biochemical functions remain mysterious, form an intricate secondar
Chemoenzymatic approaches are important for generating site-specific, chemically modified RNAs, a cornerstone for RNA structure-function correlation studies. T7 RNA polymerase (T7RNAP)-mediated in vitro transcription (IVT) of a DNA template containin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c72145b2fb88bc62b4cf901173226070
https://europepmc.org/articles/PMC6392026/
https://europepmc.org/articles/PMC6392026/
Autor:
Venkat Gopalan, Seth E. Lyon
Publikováno v:
Chembiochem : a European journal of chemical biology. 19(2)
Spectroscopic methods, which are used to establish RNA structure-function relationships, require strategies for post-synthetic, site-specific incorporation of chemical probes into target RNAs. For RNAs larger than 50 nt, the enzymatic incorporation o
Autor:
Venkat Gopalan, Seth E. Lyon
Publikováno v:
ChemBioChem. 19:114-114