Zobrazeno 1 - 10
of 217
pro vyhledávání: '"Seth A Darst"'
Autor:
Jin Young Kang, Eliza Llewellyn, James Chen, Paul Dominic B Olinares, Joshua Brewer, Brian T Chait, Elizabeth A Campbell, Seth A Darst
Publikováno v:
eLife, Vol 10 (2021)
Transcription-coupled repair (TCR) is a sub-pathway of nucleotide excision repair (NER) that preferentially removes lesions from the template-strand (t-strand) that stall RNA polymerase (RNAP) elongation complexes (ECs). Mfd mediates TCR in bacteria
Externí odkaz:
https://doaj.org/article/8123ee31e7b74c728b593caaed97ace4
Autor:
James Chen, Saumya Gopalkrishnan, Courtney Chiu, Albert Y Chen, Elizabeth A Campbell, Richard L Gourse, Wilma Ross, Seth A Darst
Publikováno v:
eLife, Vol 8 (2019)
TraR and its homolog DksA are bacterial proteins that regulate transcription initiation by binding directly to RNA polymerase (RNAP) rather than to promoter DNA. Effects of TraR mimic the combined effects of DksA and its cofactor ppGpp, but the struc
Externí odkaz:
https://doaj.org/article/e1305efd59cf4fd8a2295eb97b7ac9aa
Autor:
Hande Boyaci, James Chen, Mirjana Lilic, Margaret Palka, Rachel Anne Mooney, Robert Landick, Seth A Darst, Elizabeth A Campbell
Publikováno v:
eLife, Vol 7 (2018)
Fidaxomicin (Fdx) is an antimicrobial RNA polymerase (RNAP) inhibitor highly effective against Mycobacterium tuberculosis RNAP in vitro, but clinical use of Fdx is limited to treating Clostridium difficile intestinal infections due to poor absorption
Externí odkaz:
https://doaj.org/article/ed35d087085b40cab9518a2253b5c555
Autor:
Jin Young Kang, Paul Dominic B Olinares, James Chen, Elizabeth A Campbell, Arkady Mustaev, Brian T Chait, Max E Gottesman, Seth A Darst
Publikováno v:
eLife, Vol 6 (2017)
Coliphage HK022 Nun blocks superinfection by coliphage λ by stalling RNA polymerase (RNAP) translocation specifically on λ DNA. To provide a structural framework to understand how Nun blocks RNAP translocation, we determined structures of Escherich
Externí odkaz:
https://doaj.org/article/5854e80359a44629abd62f3c1fc151e5
Autor:
Elizabeth A Hubin, Allison Fay, Catherine Xu, James M Bean, Ruth M Saecker, Michael S Glickman, Seth A Darst, Elizabeth A Campbell
Publikováno v:
eLife, Vol 6 (2017)
RbpA and CarD are essential transcription regulators in mycobacteria. Mechanistic analyses of promoter open complex (RPo) formation establish that RbpA and CarD cooperatively stimulate formation of an intermediate (RP2) leading to RPo; formation of R
Externí odkaz:
https://doaj.org/article/fb7a564ed06b473b809505503645860d
Publikováno v:
eLife, Vol 4 (2015)
Initiation of transcription is a primary means for controlling gene expression. In bacteria, the RNA polymerase (RNAP) holoenzyme binds and unwinds promoter DNA, forming the transcription bubble of the open promoter complex (RPo). We have determined
Externí odkaz:
https://doaj.org/article/4268cf4aea50443fa856258e8b09015b
Publikováno v:
eLife, Vol 4 (2015)
A key point to regulate gene expression is at transcription initiation, and activators play a major role. CarD, an essential activator in Mycobacterium tuberculosis, is found in many bacteria, including Thermus species, but absent in Escherichia coli
Externí odkaz:
https://doaj.org/article/24074d1ff14e498d924354ef3c28dc71
Publikováno v:
PLoS ONE, Vol 10, Iss 3, p e0119007 (2015)
Recent X-ray crystallographic studies of Pol II in complex with the general transcription factor (GTF) IIB have begun to provide insights into the mechanism of transcription initiation. These structures have also shed light on the architecture of the
Externí odkaz:
https://doaj.org/article/85b62fd352da4ed4a6a874f270a20e7f
Publikováno v:
PLoS ONE, Vol 8, Iss 10, p e76572 (2013)
Staphylococcus aureus is a gram-positive cocci and an important human commensal bacteria and pathogen. S. aureus infections are increasingly difficult to treat because of the emergence of highly resistant MRSA (methicillin-resistant S. aureus) strain
Externí odkaz:
https://doaj.org/article/77734f0919c344ee86d6f58a402c66e9
Autor:
Natacha Opalka, Jesse Brown, William J Lane, Kelly-Anne F Twist, Robert Landick, Francisco J Asturias, Seth A Darst
Publikováno v:
PLoS Biology, Vol 8, Iss 9, p e1000483 (2010)
The Escherichia coli transcription system is the best characterized from a biochemical and genetic point of view and has served as a model system. Nevertheless, a molecular understanding of the details of E. coli transcription and its regulation, and
Externí odkaz:
https://doaj.org/article/c02ae16cbc07446e81ced5aaa6ec2ae6