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Inhibition of the lepidopteran amino acid co-transporter KAAT1 by phenylglyoxal: role of arginine 76
Publikováno v:
Insect Molecular Biology. 11:283-289
Phenylglyoxal (PGO), an arginine-modifying reagent, is an irreversible inhibitor of KAAT1-mediated leucine transport, expressed in Xenopus oocytes. The PGO effect was dose-dependent and 5 mm PGO determined a V(max) reduction to 24% of the control, co
The transport properties of the intestinal amino acid cotransporter KAAT1, heterologously expressed in Xenopus oocytes, were studied using simultaneous voltage-clamp and tritiated leucine uptake measurements. While addition of 1 mM leucine to oocytes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e61afe443a2bfcb0ba2134baffb709c5
http://hdl.handle.net/11383/1489907
http://hdl.handle.net/11383/1489907
Autor:
Antonio Peres, Sergio Vincenti, Elena Bossi, V. Franca Sacchi, Elena Centinaio, Michela Castagna, Stefano Giovannardi
Publikováno v:
Scopus-Elsevier
1. The transient and steady-state currents induced by voltage jumps in Xenopus oocytes expressing the lepidopteran amino acid co-transporter KAAT1 have been investigated by two-electrode voltage clamp. 2. KAAT1-expressing oocytes exhibited membrane c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f24456bbeff10afd8686e239aeb092c1
http://www.scopus.com/inward/record.url?eid=2-s2.0-0033559841&partnerID=MN8TOARS
http://www.scopus.com/inward/record.url?eid=2-s2.0-0033559841&partnerID=MN8TOARS