Reinvestigation of the Automated Synthesis of Stoichiometrically Conjugated Antibodies to Access High Molecular Weight Payloads and Multiplexed Conjugation via an In-Solution Trans-Tagging Process

Autor: Victor Lehot, Ondřej Lidický, Julien Most, Stéphane Erb, Igor Dovgan, Artem Osypenko, Oleksandr Koniev, Sergii Kolodych, Lenka Kotrchová, Guilhem Chaubet, Sarah Cianférani, Tomáš Etrych, Alain Wagner

Publikováno v: ACS Omega, Vol 8, Iss 43, Pp 40508-40516 (2023)

Externí odkaz: https://doaj.org/article/61547958d4c8422b851129ddc858ab8c

Thermofluorimetric Analysis (TFA) using Probes with Flexible Spacers: Application to Direct Antibody Sensing and to Antibody-Oligonucleotide (AbO) Conjugate Valency Monitoring

Autor: Amanda S.N. Kurian, Asanka Gurukandure, Igor Dovgan, Sergii Kolodych, Christopher J. Easley

Antibodies have long been recognized as clinically relevant biomarkers of disease. The onset of a disease often stimulates antibody production at low quantities, making it crucial to develop sensitive, specific, and easy-to-use antibody assay platfor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3accab86dd5ae3fdf2f1cdb23036671
https://doi.org/10.26434/chemrxiv-2023-lm9td

Automated linkage of proteins and payloads producing monodisperse conjugates

Autor: Alexandre Hentz, Margaux Riomet, Sébastien Delacroix, Oleksandr Koniev, Anthony Ehkirch, Sylvain Ursuegui, Igor Dovgan, Steve Hessmann, Alain Wagner, Frédéric Taran, Sergii Kolodych, Sarah Cianférani

Publikováno v: Chemical Science
Chemical Science, The Royal Society of Chemistry, 2020, 11 (5), pp.1210-1215. ⟨10.1039/c9sc05468e⟩
Chemical Science, 2020, 11 (5), pp.1210-1215. ⟨10.1039/c9sc05468e⟩

Controlled protein functionalization holds great promise for a wide variety of applications. However, despite intensive research, the stoichiometry of the functionalization reaction remains difficult to control due to the inherent stochasticity of th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31bf58838833fe846bc28076937a26d7
https://hal.archives-ouvertes.fr/hal-03012248

Design and Synthesis of Iminosydnones for Fast Click and Release Reactions with Cycloalkynes

Autor: Karine Porte, Margaux Riomet, Sabrina Bernard, Davide Audisio, Elodie Decuypere, Lucie Plougastel, Sergii Kolodych, Frédéric Taran

Publikováno v: Chemistry - A European Journal. 24:8535-8541

Emerging applications in the field of chemical biology are currently limited by the lack of bioorthogonal reactions allowing both removal and linkage of chemical entities on complex biomolecules. We recently discovered a novel reaction between iminos

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72253078aa97a3f93ce6d117a0a91176
https://doi.org/10.1002/chem.201801163

Bioorthogonal Click and Release Reaction of Iminosydnones with Cycloalkynes

Autor: Sabrina Bernard, Alain Wagner, Davide Audisio, Oleksandr Koniev, Sarah Bregant, Lucie Plougastel, Jijy Elyian, Elodie Decuypere, Sandra Gabillet, Sergii Kolodych, Frédéric Taran, Antoine Sallustrau, Margaux Riomet, Minh Nguyet Trinh, Ramar Arun Kumar

Publikováno v: Angewandte Chemie International Edition. 56:15612-15616

We report the discovery of a new bioorthogonal click-and-release reaction involving iminosydnones and strained alkynes. This transformation leads to two products resulting from the ligation and fragmentation of iminosydnones under physiological condi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::101bfe47ded49f18254c715e0b3185e4
https://doi.org/10.1002/anie.201708790