Zobrazeno 1 - 10
of 43
pro vyhledávání: '"Sergey V, Mashko"'
Autor:
Evgeniya A. Malykh, Liubov I. Golubeva, Ekaterina S. Kovaleva, Mikhail S. Shupletsov, Elena V. Rodina, Sergey V. Mashko, Nataliya V. Stoynova
Publikováno v:
Microorganisms, Vol 11, Iss 2, p 294 (2023)
Inorganic pyrophosphatases (PPases) catalyze an essential reaction, namely, the hydrolysis of PPi, which is formed in large quantities as a side product of numerous cellular reactions. In the majority of living species, PPi hydrolysis is carried out
Externí odkaz:
https://doaj.org/article/af964f35fadd418690d79f9aa38118d4
Autor:
Evgeniya A. Malykh, Ivan A. Butov, Anna B. Ravcheeva, Alexander A. Krylov, Sergey V. Mashko, Nataliya V. Stoynova
Publikováno v:
Microbial Cell Factories, Vol 17, Iss 1, Pp 1-15 (2018)
Abstract Background In the l-histidine (His) biosynthetic pathway of Escherichia coli, the first key enzyme, ATP-phosphoribosyltransferase (ATP-PRT, HisG), is subject to different types of inhibition. Eliminating the feedback inhibition of HisG by th
Externí odkaz:
https://doaj.org/article/06ba55d2ccd340a994d58e397ee90f61
Autor:
Juliya S. Lobanova, Natalya V. Gorshkova, Alexander A. Krylov, Nataliya V. Stoynova, Sergey V. Mashko
The combination of recipient chromosome modification due to recombineering by a linear dsDNA fragment with two types of site-specific recombination (SSR) systems, which were previously developed for Escherichia coli and called Dual-In/Out, followed b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6700bcb19adaa5db9618286632b52bb9
https://doi.org/10.21203/rs.3.rs-1089148/v1
https://doi.org/10.21203/rs.3.rs-1089148/v1
Autor:
Valeriya V. Shapovalova, Sergey V. Mashko, Alexander A. Krylov, M. S. Shupletsov, Elizaveta A. Miticheva
Publikováno v:
ACS Synthetic Biology. 9:1650-1664
Dynamic control is a distinguished strategy in modern metabolic engineering, in which inducible convergent transcription is an attractive approach for conditional gene silencing. Instead of a simple strong "reverse" (r-) promoter, a three-component a
Publikováno v:
Applied Biochemistry and Microbiology. 55:850-860
The uncoordinated expression of a target biosynthetic pathway often results in low growth and productivity for a producing strain due to the accumulation of toxic metabolic intermediates. In this study, we report an increase in isoprene accumulation
Genome engineering of the Corynebacterium glutamicum chromosome by the Extended Dual-In/Out strategy
Autor:
Juliya S, Lobanova, Natalya V, Gorshkova, Alexander A, Krylov, Nataliya V, Stoynova, Sergey V, Mashko
Publikováno v:
Journal of Microbiological Methods. 200:106555
A novel genome editing method for repeated introduction of foreign DNA, including insertion of rather large DNA fragments, into predesigned points in the Corynebacterium glutamicum chromosome was developed. The method is based on the implementation o
Autor:
Alexander A, Krylov, Valeriya V, Shapovalova, Elizaveta A, Miticheva, Mikhail S, Shupletsov, Sergey V, Mashko
Publikováno v:
ACS synthetic biology. 9(7)
Dynamic control is a distinguished strategy in modern metabolic engineering, in which inducible convergent transcription is an attractive approach for conditional gene silencing. Instead of a simple strong "reverse" (
Autor:
Nataliya V. Stoynova, Yurgis A. V. Yomantas, Elena G. Abalakina, Juliya S. Lobanova, Victor A. Mamontov, Sergey V. Mashko
Publikováno v:
Archives of Virology
The genomes of two new lytic phages of Corynebacterium glutamicum ATCC 13032, φ673 and φ674, were sequenced and annotated (GenBank: MG324353, MG324354). Electron microscopy studies of both virions revealed that taxonomically they belong to the Siph
Mu-driven transposition of recombinant mini-Mu unit DNA in the Corynebacterium glutamicum chromosome
Autor:
Sergey Vasilievich Smirnov, Irina L. Tokmakova, Natalya V. Gorshkova, Juliya S. Lobanova, Valerii Z. Akhverdyan, Sergey V. Mashko, Alexander A. Krylov
Publikováno v:
Applied Microbiology and Biotechnology
A dual-component Mu-transposition system was modified for the integration/amplification of genes in Corynebacterium. The system consists of two types of plasmids: (i) a non-replicative integrative plasmid that contains the transposing mini-Mu(LR) uni
Publikováno v:
Applied Biochemistry and Microbiology. 53:733-753
Quantitatively characterizing the intracellular carbon flux distribution provides useful information for both fundamental and applied investigations into the cellular metabolism at the system level, such as the roles of different metabolic pathways a