Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Serdar Aykent"'
Autor:
Bilikallahalli K. Muralidhara, Michael R. Schlittler, Qian Wang, Charles W. Demarest, Nathan A. Lacher, Serdar Aykent
Publikováno v:
Journal of Separation Science. 33:2671-2680
A rapid reversed-phase HPLC separation of recombinant human immunoglobulin gamma 2 (IgG2) disulfide isomers using columns packed with superficially porous particles is reported. Under optimal conditions, a separation of monoclonal IgG2 disulfide isom
Autor:
Serdar Aykent, Oliphant Theodore Lawrence, Kristine Wells, Nathan A. Lacher, Sandra Lightle, Vesselin Mitaksov, James F. Zobel
Publikováno v:
Protein Science. 19:753-762
Human IgG2 antibodies may exist in at least three distinct structural isomers due to disulfide shuffling within the upper hinge region. Antibody interactions with Fc gamma receptors and the complement component C1q contribute to immune effector funct
Autor:
Rachel K. Roberts, Michael R. Schlittler, Serdar Aykent, Qian Wang, Nathan A. Lacher, Melissa Thompson, Charles W. Demarest, Heidi J. Holovics
Publikováno v:
ELECTROPHORESIS. 31:448-458
A CGE method for monitoring the disulfide isomer distribution characteristic of IgG2 MAbs is presented. Disulfide heterogeneity of MAbs has been studied using various chromatographic and electrophoretic methods. Although CGE operates using a differen
Publikováno v:
Biochemistry. 34:6540-6551
Interleukin-3 (IL-3) is a cytokine which stimulates the proliferation and differentiation of hematopoietic progenitors into multiple cell lineages. The 1H, 15N, and 13C NMR resonances of a recombinant human IL-3 variant (SC-65369) have been assigned
Autor:
Serdar Aykent, K.L. Duffin, N.M. Kimack, G.S. Bild, Gwen G. Krivi, M.J. Bourner, L.J. Askonas, C.P. Rodi, P.M. Sullivan, James K. Gierse, V.A. Luckow
Publikováno v:
Protein Expression and Purification. 4:358-366
Leukotrienes constitute a group of bioactive compounds derived from arachidonic acid which play important roles in immediate hypersensitivity and inflammation. Leukotriene A 4 hydrolase (LTA 4 H) is an epoxide hydrolase, catalyzing the hydration of L
Autor:
Qian, Wang, Nathan A, Lacher, Bilikallahalli K, Muralidhara, Michael R, Schlittler, Serdar, Aykent, Charles W, Demarest
Publikováno v:
Journal of separation science. 33(17-18)
A rapid reversed-phase HPLC separation of recombinant human immunoglobulin gamma 2 (IgG2) disulfide isomers using columns packed with superficially porous particles is reported. Under optimal conditions, a separation of monoclonal IgG2 disulfide isom
Autor:
Sandra, Lightle, Serdar, Aykent, Nathan, Lacher, Vesselin, Mitaksov, Kristine, Wells, James, Zobel, Theodore, Oliphant
Publikováno v:
Protein science : a publication of the Protein Society. 19(4)
Human IgG2 antibodies may exist in at least three distinct structural isomers due to disulfide shuffling within the upper hinge region. Antibody interactions with Fc gamma receptors and the complement component C1q contribute to immune effector funct
Publikováno v:
Journal of Biological Chemistry. 265:15253-15260
The NGFI-A gene, which encodes a zinc finger protein with a predicted molecular mass of congruent to 54 kDa, is rapidly activated in PC12 cells by nerve growth factor (NGF). As a transcription factor, NGFI-A is a potentially important mediator of the
Publikováno v:
Biochemistry. 30(6)
The disulfide pairings of mouse and human interleukin 4 (IL-4) proteins have been determined. The purified proteins, synthesized by recombinant DNA technology, are fully active as judged by their ability to stimulate an appropriate biological respons
Publikováno v:
Biochemistry. 29(12)
Steady-state kinetic analysis of human renin demonstrates the histidine proximal to the substrate scissile peptide bond contributes to the unique specificity and pH dependence of this aspartyl protease. Recombinant human renal renin purified from mam