Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Semra Alemdar"'
Autor:
Semra Alemdar, Andreas Kirschning, Sascha Beutel, Thomas Scheper, Jan C. L. König, Katja Seidel
Publikováno v:
Eng Life Sci
Terpene synthases are the key enzymes in terpene biosynthesis that provide a structurally complex and highly diverse product spectrum. A suitable and reliable analytical assay is indispensable to measure terpene synthase activity accurately and preci
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::643d21c7bc5c15be1959de9963e95aba
https://doi.org/10.1002/elsc.201800019
https://doi.org/10.1002/elsc.201800019
Autor:
Jan C. L. König, Steffen Hartwig, Thore Frister, Semra Alemdar, Sascha Beutel, Thomas Scheper
Publikováno v:
Applied Biochemistry and Biotechnology 178 (2016), Nr. 3
The α-humulene synthase from Zingiber zerumbet Smith was expressed as a polyhistidine-tagged protein in an E. coli BL21(DE3) strain. Induction time and inductor (isopropyl-β-D-thiogalactopyranoside) concentration were optimized. The enzyme was succ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42d51b4a3978a9049b9c9aa1ac079e3c
https://doi.org/10.1007/s12010-015-1888-4
https://doi.org/10.1007/s12010-015-1888-4
Autor:
Thomas Scheper, Katharina Schnatz, Semra Alemdar, Thore Frister, Steffen Hartwig, Sascha Beutel, Laura Thöns
Publikováno v:
Applied Biochemistry and Biotechnology 176 (2015), Nr. 8
The patchoulol synthase (PTS) is a multi-product sesquiterpene synthases which is the central enzyme for biosynthesis of patchouli essential oil in the patchouli plant. Sesquiterpene synthases catalyse the formation of various complex carbon backbone
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e99030cd3a656b9fa8bc98007838bd5b
https://doi.org/10.1007/s12010-015-1707-y
https://doi.org/10.1007/s12010-015-1707-y
Publikováno v:
Biochemical and Biophysical Research Communications 458 (2015), Nr. 4
An uncharacterized plant cDNA coding for a polypeptide presumably having sesquiterpene synthase activity, was expressed in soluble and active form. Two expression strategies were evaluated in Escherichia coli. The enzyme was fused to a highly soluble
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63dddfed8ea44cbe42ba365b445f2815
https://doi.org/10.1016/j.bbrc.2015.02.053
https://doi.org/10.1016/j.bbrc.2015.02.053
Autor:
Semra Alemdar, Thomas Scheper, Sascha Beutel, Thore Frister, Steffen Hartwig, Jan C. L. König
Publikováno v:
Eng Life Sci
Zerumbone is a sesquiterpene ketone with potent anti-cancerogenic activities, produced in several ginger species of the Zingiberaceae familiy. We have investigated the biotechnological production of α-humulene, a precursor of zerumbone. By implement
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cfa92f0ffc60bd0e13143fd6420a04cb
https://europepmc.org/articles/PMC6999591/
https://europepmc.org/articles/PMC6999591/
Autor:
Semra Alemdar, Ralf G. Berger, Thore Frister, Ulrich Krings, Zhaopeng Li, T. Scheper, Steffen Hartwig, Sascha Beutel
Publikováno v:
Protein expression and purification. 97
Probing a cDNA library extracted from Pogostemon cablin (Indian Patchouli) with gene specific primers, a variant of patchoulol synthase PTS (GenBank acc. No.: AY508730) was amplified, cloned, and sequenced. The amino acid sequence deduced from the cl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::763c0d766602ca332efaf27e6626d404
https://pubmed.ncbi.nlm.nih.gov/24576659
https://pubmed.ncbi.nlm.nih.gov/24576659
Publikováno v:
Chemie Ingenieur Technik. 88:1395-1395
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::70b4cf27c1301fb60f1805523ca772f4
https://doi.org/10.1002/cite.201650082
https://doi.org/10.1002/cite.201650082