A multivalent mRNA-LNP vaccine protects against Clostridioides difficile infection.

Autor: Alameh MG; Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Penn Institute for RNA Innovation, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.; Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA., Semon A; Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Division of Protective Immunity, Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Institute for Immunology and Immune Health, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Bayard NU; Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Division of Protective Immunity, Children's Hospital of Philadelphia, Philadelphia, PA, USA., Pan YG; Division of Infectious Disease, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Dwivedi G; Division of Infectious Disease, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Knox J; Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Glover RC; Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Division of Protective Immunity, Children's Hospital of Philadelphia, Philadelphia, PA, USA., Rangel PC; Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Division of Protective Immunity, Children's Hospital of Philadelphia, Philadelphia, PA, USA., Tanes C; Division of Gastroenterology, Hepatology, and Nutrition, The Children's Hospital of Philadelphia, Philadelphia, PA, USA., Bittinger K; Division of Gastroenterology, Hepatology, and Nutrition, The Children's Hospital of Philadelphia, Philadelphia, PA, USA.; The Center for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA., She Q; Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Division of Gastroenterology, Hepatology, and Nutrition, The Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.; Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA., Hu H; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA., Bonam SR; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA., Maslanka JR; Institute for Immunology and Immune Health, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA., Planet PJ; The Center for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.; Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Division of Pediatric Infectious Diseases, Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Comparative Genomics, American Museum of Natural History, New York, NY, USA., Moustafa AM; Division of Gastroenterology, Hepatology, and Nutrition, The Children's Hospital of Philadelphia, Philadelphia, PA, USA.; The Center for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Davis B; Division of Infectious Disease, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Chevrier A; Chemical Engineering Department, Polytechnique Montreal, Montreal, QC, Canada., Beattie M; Acuitas Therapeutics, Vancouver, British Columbia, Canada., Ni H; Acuitas Therapeutics, Vancouver, British Columbia, Canada., Blizard G; Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Furth EE; Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Mach RH; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Lavertu M; Chemical Engineering Department, Polytechnique Montreal, Montreal, QC, Canada., Sellmyer MA; Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Tam Y; Acuitas Therapeutics, Vancouver, British Columbia, Canada., Abt MC; Institute for Immunology and Immune Health, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA., Weissman D; Penn Institute for RNA Innovation, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.; Division of Infectious Disease, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Zackular JP; Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Penn Institute for RNA Innovation, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.; Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Division of Protective Immunity, Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Institute for Immunology and Immune Health, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; The Center for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA.

Publikováno v: Science (New York, N.Y.) [Science] 2024 Oct 04; Vol. 386 (6717), pp. 69-75. Date of Electronic Publication: 2024 Oct 03.

Live Organoid Cyclic Imaging.

Autor: Reynolds DE; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, 19104, USA., Sun Y; Department of Neuroscience, Mahoney Institute for Neurosciences, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA., Wang X; Department of Neuroscience, Mahoney Institute for Neurosciences, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA., Vallapureddy P; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, 19104, USA., Lim J; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, 19104, USA., Pan M; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, 19104, USA., Fernandez Del Castillo A; Department of Biochemistry & Molecular Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA., Carlson JCT; Center for Systems Biology, Massachusetts General Hospital, Boston, MA, 02114, USA.; Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA., Sellmyer MA; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA., Nasrallah M; GBM Translational Center of Excellence, Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA, 19104, USA.; Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA., Binder Z; GBM Translational Center of Excellence, Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA, 19104, USA.; Center for Cellular Immunotherapies, University of Pennsylvania, Philadelphia, PA, 19104, USA.; Department of Neurosurgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA., O'Rourke DM; GBM Translational Center of Excellence, Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA, 19104, USA.; Center for Cellular Immunotherapies, University of Pennsylvania, Philadelphia, PA, 19104, USA.; Department of Neurosurgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA., Ming GL; Department of Neuroscience, Mahoney Institute for Neurosciences, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.; Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.; Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.; Institute for Regenerative Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA., Song H; Department of Neuroscience, Mahoney Institute for Neurosciences, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.; GBM Translational Center of Excellence, Abramson Cancer Center, University of Pennsylvania, Philadelphia, PA, 19104, USA.; Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.; Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.; The Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA., Ko J; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, 19104, USA.; Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.

Publikováno v: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2024 Apr; Vol. 11 (14), pp. e2309289. Date of Electronic Publication: 2024 Feb 07.

A genetically encoded protein tag for control and quantitative imaging of CAR T cell therapy.

Autor: Lee IK; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA., Sharma N; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA., Noguera-Ortega E; Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA., Liousia M; Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA., Baroja ML; Center for Cellular Immunotherapies, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA., Etersque JM; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA., Pham J; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA., Sarkar S; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA., Carreno BM; Center for Cellular Immunotherapies, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA., Linette GP; Center for Cellular Immunotherapies, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA., Puré E; Department of Biomedical Sciences, University of Pennsylvania, Philadelphia, PA 19104, USA., Albelda SM; Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Center for Cellular Immunotherapies, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA., Sellmyer MA; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA; Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: mark.sellmyer@pennmedicine.upenn.edu.

Publikováno v: Molecular therapy : the journal of the American Society of Gene Therapy [Mol Ther] 2023 Dec 06; Vol. 31 (12), pp. 3564-3578. Date of Electronic Publication: 2023 Nov 03.

Visualizing Bacterial Infections With Novel Targeted Molecular Imaging Approaches.

Autor: Chen X; Center for Infection and Inflammation Imaging Research, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.; Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.; Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Gallagher F; Department of Radiology, University of Cambridge, Cambridge, United Kingdom., Sellmyer MA; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA., Ordonez AA; Center for Infection and Inflammation Imaging Research, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.; Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.; Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Kjaer A; Department of Clinical Physiology and Nuclear Medicine and Cluster for Molecular Imaging, Copenhagen University Hospital-Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.; Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark., Ohliger M; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California, USA., Wilson DM; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California, USA., Jain SK; Center for Infection and Inflammation Imaging Research, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.; Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.; Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Publikováno v: The Journal of infectious diseases [J Infect Dis] 2023 Oct 03; Vol. 228 (Suppl 4), pp. S249-S258.

A microbiome-dependent gut-brain pathway regulates motivation for exercise.

Autor: Dohnalová L; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Obesity, Diabetes and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Molecular Bio Science, Goethe University Frankfurt, and LOEWE Center for Translational Biodiversity Genomics, Frankfurt, Germany., Lundgren P; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Obesity, Diabetes and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Carty JRE; Department of Biology, University of Pennsylvania, Philadelphia, PA, USA., Goldstein N; Department of Biology, University of Pennsylvania, Philadelphia, PA, USA., Wenski SL; Institute for Molecular Bio Science, Goethe University Frankfurt, and LOEWE Center for Translational Biodiversity Genomics, Frankfurt, Germany., Nanudorn P; Institute for Molecular Bio Science, Goethe University Frankfurt, and LOEWE Center for Translational Biodiversity Genomics, Frankfurt, Germany., Thiengmag S; Institute for Molecular Bio Science, Goethe University Frankfurt, and LOEWE Center for Translational Biodiversity Genomics, Frankfurt, Germany., Huang KP; Monell Chemical Senses Center, Philadelphia, PA, USA., Litichevskiy L; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Obesity, Diabetes and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Descamps HC; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Obesity, Diabetes and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Chellappa K; Institute for Obesity, Diabetes and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Department of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Glassman A; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Obesity, Diabetes and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Kessler S; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Obesity, Diabetes and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Kim J; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Obesity, Diabetes and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Cox TO; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Obesity, Diabetes and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Dmitrieva-Posocco O; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Wong AC; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Allman EL; Department of Biochemistry and Molecular Biology and Department of Veterinary and Biomedical Sciences, the Pennsylvania State University, University Park, PA, USA., Ghosh S; Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Sharma N; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Sengupta K; Department of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Pennsylvania Muscle Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Cornes B; The Jackson Laboratory, Bar Harbor, ME, USA., Dean N; Hoss Technology, New York, NY, USA., Churchill GA; The Jackson Laboratory, Bar Harbor, ME, USA., Khurana TS; Department of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Pennsylvania Muscle Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Sellmyer MA; Department of Radiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., FitzGerald GA; Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Translational Medicine and Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Patterson AD; Department of Biochemistry and Molecular Biology and Department of Veterinary and Biomedical Sciences, the Pennsylvania State University, University Park, PA, USA., Baur JA; Institute for Obesity, Diabetes and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Department of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Alhadeff AL; Monell Chemical Senses Center, Philadelphia, PA, USA.; Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Helfrich EJN; Institute for Molecular Bio Science, Goethe University Frankfurt, and LOEWE Center for Translational Biodiversity Genomics, Frankfurt, Germany., Levy M; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Betley JN; Institute for Obesity, Diabetes and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Department of Biology, University of Pennsylvania, Philadelphia, PA, USA., Thaiss CA; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. thaiss@pennmedicine.upenn.edu.; Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. thaiss@pennmedicine.upenn.edu.; Institute for Obesity, Diabetes and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. thaiss@pennmedicine.upenn.edu.

Publikováno v: Nature [Nature] 2022 Dec; Vol. 612 (7941), pp. 739-747. Date of Electronic Publication: 2022 Dec 14.