Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells

Autor: Greta Bigelyte, Joshua K. Young, Tautvydas Karvelis, Karolina Budre, Rimante Zedaveinyte, Vesna Djukanovic, Elizabeth Van Ginkel, Sushmitha Paulraj, Stephen Gasior, Spencer Jones, Lanie Feigenbutz, Grace St. Clair, Pierluigi Barone, Jennifer Bohn, Ananta Acharya, Gina Zastrow-Hayes, Selgar Henkel-Heinecke, Arunas Silanskas, Ralf Seidel, Virginijus Siksnys

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021)

Miniature Cas12f editing systems are well suited for in vivo editing applications. Here the authors characterize the intrinsic activity of SpCas12f1 in plant and animal cells.

Externí odkaz: https://doaj.org/article/688a63ea2c9f45e084a40d762048f444

Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells

Autor: Karolina Budre, Stephen L. Gasior, Selgar Henkel-Heinecke, Tautvydas Karvelis, Ralf Seidel, Virginijus Siksnys, Vesna Djukanovic, Rimante Zedaveinyte, Spencer Jones, Sushmitha Paulraj, Arunas Silanskas, Elizabeth Van Ginkel, Grace St. Clair, Greta Bigelyte, Pierluigi Barone, Gina Zastrow-Hayes, Ananta Acharya, Jennifer A. Bohn, Joshua K. Young, Lanie Feigenbutz

Publikováno v: Nature communications, Berlin : Nature research, 2021, vol. 12, iss. 1, art. no. 6191, p. [1-8]
Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021)

Class 2 CRISPR systems are exceptionally diverse, nevertheless, all share a single effector protein that contains a conserved RuvC-like nuclease domain. Interestingly, the size of these CRISPR-associated (Cas) nucleases ranges from >1000 amino acids

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a52bb3de9810691d01aee8bf73372b5d