Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Seiji Ishiyama"'
Autor:
Keiko Higuchi, Syoko Ebihara, Mashahiro Ikeda, Yumiko Hosaka, Toshiko Mochizuki, Hironobu Okazaki, Akihiro Usami, Yukiko Ishikawa, Seiji Ishiyama, Hidekazu Nagaya, Teruyuki Koyama, Yoshimi Asano, Takeshi Yamamoto, Chiaki Enomoto, Mutsumi Sugai, Yoneko Tobita
Publikováno v:
Journal of Biochemistry. 149:219-227
Using a hybrid baculovirus system, we compared the expression of 45 recombinant proteins from six categories using two models: silkworm (larvae and pupae) and an Sf9 cell line. A total of 45 proteins were successfully expressed; preparation of hybrid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e3720caf8d6d51642c1bd5bc6eb450f
https://doi.org/10.1093/jb/mvq138
https://doi.org/10.1093/jb/mvq138
Autor:
Seiji Ishiyama, Toshiki Tamura, Hideki Sezutsu, Tatsuji Seki, Kazuei Mita, Kazuhito Fujiyama, Akihiro Usami, Takahiro Okada
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 71:1626-1635
Beta-N-acetylglucosaminidase is a major glycosidase involved in several physiological processes, such as fertilization, metamorphosis, glycoconjugate degradation, and glycoprotein biosynthesis in insects. A search using the Bombyx mori cDNA database
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b460c663c7cb26ad69bd642d0d34f560
https://doi.org/10.1271/bbb.60705
https://doi.org/10.1271/bbb.60705
Autor:
Seiji Ishiyama, Masahiro Ikeda
Publikováno v:
Biotechnology letters. 32(11)
Some recombinant proteins expressed by baculovirus expression vector systems (BEVS) aggregate because the BEVS can produce large amounts of protein late during infection, when post-translational modification and protein quality control mechanisms are
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1b1757d97f0b55644b5ec6af02d73da
https://pubmed.ncbi.nlm.nih.gov/20821250
https://pubmed.ncbi.nlm.nih.gov/20821250
Autor:
Toshiki Tamura, Akihiro Usami, Tsuyoshi Nomura, Masahiro Ikeda, Takahiro Okada, Seiji Ishiyama, Mita Kazuhide, Kazuhito Fujiyama
Publikováno v:
Journal of bioscience and bioengineering. 110(4)
In insects, β-N-acetylglucosaminidase (GlcNAcase) participates in critical physiological processes such as fertilization, metamorphosis, and glycoconjugate degradation. Insects produce glycoproteins carrying paucimannosidic-type N-glycans, the termi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b33520c6fedf791520aa82b707a37625
https://pubmed.ncbi.nlm.nih.gov/20547376
https://pubmed.ncbi.nlm.nih.gov/20547376
Autor:
Hiroshi Imagawa, Masatoshi Tanigawa, Shigeo Sugita, Noriyuki Saeki, Takeo Sugiura, Akira Kuwano, Seiji Ishiyama, Akihiko Uchiyama, Toshimichi Kanaya
Publikováno v:
Journal of virological methods. 98(1)
The hemagglutinin (HA) protein of an equine influenza strain, A/equine/La Plata/1/93 (LP/93), was produced using a baculovirus expression system. Silkworm larvae inoculated with recombinant baculovirus expressed high quantities of the HA protein whic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::212eb25e9baaa89de06536bf68ed9410
https://pubmed.ncbi.nlm.nih.gov/11543878
https://pubmed.ncbi.nlm.nih.gov/11543878
Autor:
Kenji Murakami, Akihiko Uchiyama, Takehiro Kokuho, Yasuyuki Mori, Hiroshi Sentsui, Takashi Yada, Masatoshi Tanigawa, Akira Kuwano, Hidekazu Nagaya, Seiji Ishiyama, Hiroki Kaki, Yuichi Yokomizo, Shigeki Inumaru
Publikováno v:
Cytokine. 13(1)
The full-length bovine interferon-gamma (bIFN-gamma) cDNA, including the secretion signal peptide coding region was recloned into baculovirus transfer vectors pAcYM1 and pBm050. These vectors were co-transfected with Autographa californica nuclear po
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2465fa9712ab95caeaee724cf6c1eae
https://pubmed.ncbi.nlm.nih.gov/11145838
https://pubmed.ncbi.nlm.nih.gov/11145838