Reversal of axonal growth defects in an extraocular fibrosis model by engineering the kinesin–microtubule interface

Autor: Itsushi Minoura, Hiroko Takazaki, Rie Ayukawa, Chihiro Saruta, You Hachikubo, Seiichi Uchimura, Tomonobu Hida, Hiroyuki Kamiguchi, Tomomi Shimogori, Etsuko Muto

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-11 (2016)

How mutations in β3-tubulin cause axonal growth defects in congenital fibrosis of the extraocular muscles type 3 remains elusive. Minoura et al. develop a model system using recombinant human tubulin that demonstrates a link between tubulin mutation

Externí odkaz: https://doaj.org/article/e1f2163676ed4f4f9ecb892722c22875

GTP-dependent formation of straight tubulin oligomers leads to microtubule nucleation

Autor: Shinji Kamimura, Rie Ayukawa, Seigo Iwata, Benoît Gigant, Kien Xuan Ngo, Hiroshi Imai, Masahito Hayashi, Mikako Shirouzu, Itsushi Minoura, Ken Sekimoto, Etsuko Muto, Hideki Shigematsu, Tsukasa Makino, Seiichi Uchimura

Publikováno v: Journal of Cell Biology
Journal of Cell Biology, 2021, 220 (e202007033), ⟨10.1083/jcb.202007033⟩
Journal of Cell Biology, 2021, 220, ⟨10.1083/jcb.202007033⟩
Journal of Cell Biology, Rockefeller University Press, 2021, 220, ⟨10.1083/jcb.202007033⟩
The Journal of Cell Biology
'Journal of Cell Biology ', vol: 220, pages: e202007033-1-e202007033-19 (2021)

Ayukawa, Iwata, Imai, et al. visualize the early intermediates in the pathway of spontaneous nucleation of microtubules by using rapid flush negative stain electron microscopy. This study demonstrates that the formation of straight tubulin oligomers

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6cb7d0964587e9adf6460d548336fe3e
https://hal.science/hal-03141626

Affinity Purification and Characterization of Functional Tubulin from Cell Suspension Cultures of Arabidopsis and Tobacco

Autor: Satoshi Fujita, Seiichi Uchimura, Taku Demura, Etsuko Muto, Takashi Hotta, Takashi Hashimoto, Masahiro Noguchi

Publikováno v: Plant Physiology. 170:1189-1205

Microtubules assemble into several distinct arrays that play important roles in cell division and cell morphogenesis. To decipher the mechanisms that regulate the dynamics and organization of this versatile cytoskeletal component, it is essential to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2042c7812834ccb17e8a7bcfaf3939b2
https://doi.org/10.1104/pp.15.01173

The bidirectional depolymerizer MCAK generates force by disassembling both microtubule ends

Autor: Yusuke Oguchi, Shin'ichi Ishiwata, Takashi Ohki, Seiichi Uchimura, Sergey V. Mikhailenko

Publikováno v: Nature Cell Biology. 13:846-852

During cell division the replicated chromosomes are segregated precisely towards the spindle poles. Although many cellular processes involving motility require ATP-fuelled force generation by motor proteins, most models of the chromosome movement inv

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6bc258ec99de31cd405898e5056022ea
https://doi.org/10.1038/ncb2256

A flipped ion pair at the dynein-microtubule interface is critical for dynein motility and ATPase activation

Autor: Hiroko Takazaki, Keiichi Namba, You Hachikubo, Takashi Fujii, Seiichi Uchimura, Takahide Kon, Itsushi Minoura, Rie Ayukawa, Etsuko Muto, Yosuke Nishikawa, Genji Kurisu, Kazuo Sutoh

Publikováno v: The Journal of Cell Biology

Salt bridges at the dynein–microtubule interface couple microtubule binding to ATPase activation and thereby control the directional movement of dynein
Dynein is a motor protein that moves on microtubules (MTs) using the energy of adenosine tr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a7b849dea5a371329dca80c4840a6ef
https://pubmed.ncbi.nlm.nih.gov/25583999