Zobrazeno 1 - 10
of 207
pro vyhledávání: '"Seiichi TOKI"'
Autor:
Kazuhiro Ishibashi, Satoru Sukegawa, Masaki Endo, Naho Hara, Osamu Nureki, Hiroaki Saika, Seiichi Toki
Publikováno v:
Frontiers in Plant Science, Vol 15 (2024)
Because virus vectors can spread systemically autonomously, they are powerful vehicles with which to deliver genome-editing tools into plant cells. Indeed, a vector based on a positive-strand RNA virus, potato virus X (PVX), harboring SpCas9 and its
Externí odkaz:
https://doaj.org/article/294dd67f98a94dbeaf99f1b92fd07bf5
Autor:
Mitsuru Nishiguchi, Norihiro Futamura, Masaki Endo, Masafumi Mikami, Seiichi Toki, Shin-Ichiro Katahata, Yasunori Ohmiya, Ken-ichi Konagaya, Yoshihiko Nanasato, Toru Taniguchi, Tsuyoshi Emilio Maruyama
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-11 (2023)
Abstract Sugi (Cryptomeria japonica D. Don) is an economically important coniferous tree in Japan. However, abundant sugi pollen grains are dispersed and transported by the wind each spring and cause a severe pollen allergy syndrome (Japanese cedar p
Externí odkaz:
https://doaj.org/article/f9bd79447bd94ea18df31a0743295422
Publikováno v:
Frontiers in Genome Editing, Vol 5 (2023)
Cas9 derived from Streptococcus pyogenes (SpCas9) is used widely in genome editing using the CRISPR-Cas system due to its high activity, but is a relatively large molecule (1,368 amino acid (a.a.) residues). Recently, targeted mutagenesis in human ce
Externí odkaz:
https://doaj.org/article/468d648b81f545dd92abc7cc4ad48ef5
Publikováno v:
Rice, Vol 15, Iss 1, Pp 1-10 (2022)
Abstract Genome editing technology can be used for gene engineering in many organisms. A target metabolite can be fortified by the knockout and modification of target genes encoding enzymes involved in catabolic and biosynthesis pathways, respectivel
Externí odkaz:
https://doaj.org/article/375043d6bb424bb9b7d88615f2952c37
Autor:
Ikuko Nakajima, Hiroyuki Kawahigashi, Chikako Nishitani, Akifumi Azuma, Takashi Haji, Seiichi Toki, Masaki Endo
Publikováno v:
PLoS ONE, Vol 18, Iss 6, p e0286698 (2023)
Transposition of transposable elements affect expression levels, splicing and epigenetic status, and function of genes located in, or near, the inserted/excised locus. For example, in grape, presence of the Gret1 retrotransposon in the promoter regio
Externí odkaz:
https://doaj.org/article/915c201679184566b9299a5a0fc93d1a
Autor:
Yuki Yanagawa, Yuma Suenaga, Yusuke Iijima, Masaki Endo, Naoko Sanada, Etsuko Katoh, Seiichi Toki, Akitoshi Okino, Ichiro Mitsuhara
Publikováno v:
PLoS ONE, Vol 18, Iss 2 (2023)
Previously, we developed a technique to introduce a superfolder green fluorescent protein (sGFP) fusion protein directly into plant cells using atmospheric-pressure plasma. In this study, we attempted genome editing using CRISPR/Cas9 (clustered regul
Externí odkaz:
https://doaj.org/article/beda2e09274143ca87e643c0d7fb34fe
Publikováno v:
Rice, Vol 13, Iss 1, Pp 1-12 (2020)
Abstract Background Gamma-aminobutyric acid (GABA) is a non-protein amino acid present in all living things. GABA is mainly synthesized from glutamate by glutamate decarboxylase (GAD). In plants the enzymatic activity of GAD is activated by Ca2+/calm
Externí odkaz:
https://doaj.org/article/1ffde60904a242b6b64947ac6fadd766
Publikováno v:
Rice, Vol 12, Iss 1, Pp 1-5 (2019)
Abstract Background β-carotene (provitamin A) is an important target for biofortification of crops as a potential solution to the problem of vitamin A deficiency that is prevalent in developing countries. A previous report showed that dominant expre
Externí odkaz:
https://doaj.org/article/38d6da82b1b649d1bfd182e22011a355
Autor:
Hayato Yoshioka, Keiko Kimura, Yuko Ogo, Namie Ohtsuki, Ayako Nishizawa-Yokoi, Hironori Itoh, Seiichi Toki, Takeshi Izawa
Publikováno v:
Frontiers in Plant Science, Vol 12 (2021)
Flowering is an important biological process through which plants determine the timing of reproduction. In rice, florigen mRNA is induced more strongly when the day length is shorter than the critical day length through recognition of 30-min differen
Externí odkaz:
https://doaj.org/article/12cdc3abd6104fcd8dd75a3b62304255
Publikováno v:
Frontiers in Genome Editing, Vol 3 (2021)
Externí odkaz:
https://doaj.org/article/ea1185c9bcb541a38c6e794fcc999699