Zobrazeno 1 - 10
of 61
pro vyhledávání: '"Seiga Itoh"'
Autor:
Yoshihisa Kuwana, Mamoru Hasegawa, Seiga Itoh, Hiromasa Miyaji, Kikuko Funayama, Hajime Yoshida
Publikováno v:
Cytotechnology. 37:133-141
The hybridoma cell line KM50 originally produces a monoclonal antibody at a concentration of ∼40 mg ml-1 in ascites. To investigate the possibility to apply this expression system to the production of useful proteins, the cDNA encoding human granul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::03a5d4bc4a81fd7c0676dee7b117ad52
https://doi.org/10.1023/a:1020585320775
https://doi.org/10.1023/a:1020585320775
Autor:
Seiji Sato, Tamio Mizukami, Mamoru Hasegawa, Mitsuo Satoh, Tatsunari Nishi, Hiromasa Miyaji, Seiga Itoh
Publikováno v:
Cytotechnology. 18:167-172
We have compared the level of expression of several enhancer/promoters in human lymphoblastoid Namalwa KJM-1 cells when fused to a common reporter gene. A cassette containing the pro-urokinase (pro-UK) coding sequence followed by the rabbit beta-glob
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5750fa1ec60a77a63b96152a6c4c77db
https://doi.org/10.1007/bf00767764
https://doi.org/10.1007/bf00767764
Publikováno v:
Nippon Nōgeikagaku Kaishi. 70:987-992
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::271783583cb13b1e505c6525784dce7b
https://doi.org/10.1271/nogeikagaku1924.70.987
https://doi.org/10.1271/nogeikagaku1924.70.987
Publikováno v:
Cytotechnology. 13:79-88
Human pro-urokinase (pro-UK) gene was engineered for expression in mammalian cells. The stability of recombinant pro-UKs produced by two kinds of cells, Chinese hamster ovary (CHO) and human lymphoblastoid Namalwa KJM-1 cells, were compared. The pro-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49503fa783a85a53d0e528273c2d02ee
https://doi.org/10.1007/bf00749934
https://doi.org/10.1007/bf00749934
Autor:
Seiga Itoh, Tatsunari Nishi
Publikováno v:
Trends in Glycoscience and Glycotechnology. 4:336-344
組織型プラスミノーゲン活性化因子、エリスロポイエチン、そして顆粒球-マクロファージ・コロニー刺激因子などの医療用蛋白質に関する最近の研究により、それらに付いている糖鎖は
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9366a9f9620cd2a3f96563987d79fe7a
https://doi.org/10.4052/tigg.4.336
https://doi.org/10.4052/tigg.4.336
Publikováno v:
Biotechnology Letters. 13:851-856
The salmon growth hormone gene was introduced into the cyanobactriumAgmenellum quadruplicatum PR-6 by plasmid transformation. The gene expressed the hormone under thetrp promoter ofEscherichia. coli. The amount was estimated to be approximately 0.1%
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::34c3f51af2c11e26f1de67a98bbff32c
https://doi.org/10.1007/bf01022085
https://doi.org/10.1007/bf01022085
Autor:
Hiromasa Miyaji, Shinji Hosoi, Nobuo Fujiyoshi, Seiga Itoh, Tsukasa Kurimoto, Seiji Sato, Mitsuo Satoh, Akira Mihara
Publikováno v:
Cytotechnology. 5:17-34
We investigated the basic technology of cell culture conditions for production of useful substances such as cytokines, and related proteins produced by Namalwa cells. Namalwa cells (Klein, 1972), human B lymphoblastoid cells, were used for large scal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9baaeffe1c1fbd3f5252752d74c2315d
https://doi.org/10.1007/bf00573878
https://doi.org/10.1007/bf00573878
Publikováno v:
Cytotechnology. 4:173-180
We previously reported the expression of human beta-interferon (beta-IFN) (Miyaji et al., 1989) and human lymphotoxin (Miyaji et al., 1990) in Namalwa KJM-1 cells adapted to serum-free medium. To establish an efficient gene expression system, a dihyd
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b04de2e98f955a65cfd6cf72ed880e7
https://doi.org/10.1007/bf00365098
https://doi.org/10.1007/bf00365098
Publikováno v:
Cytotechnology. 4:39-43
A Namalwa cell line, KJM-1, which was adapted to serum-free medium is thought to be a good host cell line for recombinant DNA technology. We previously reported the expression of human beta-interferon (beta-IFN) in Namalwa KJM-1 (Miyaji, 1989a). The
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f71052fd9d63855877083c7985a78daf
https://doi.org/10.1007/bf00148809
https://doi.org/10.1007/bf00148809