A Salmonella typhimurium ghost vaccine induces cytokine expression in vitro and immune responses in vivo and protects rats against homologous and heterologous challenges.

Autor: Nagarajan Vinod, Han Byul Noh, Sung Oh, Seongmi Ji, Hyun Jung Park, Ki-Sung Lee, Sei Chang Kim, Han-Oh Park, Joo-Sung Yang, Chang Won Choi

Publikováno v: PLoS ONE, Vol 12, Iss 9, p e0185488 (2017)

Salmonella enteritidis and Salmonella typhimurium are important food-borne bacterial pathogens, which are responsible for diarrhea and gastroenteritis in humans and animals. In this study, S. typhimurium bacterial ghost (STG) was generated based on m

Externí odkaz: https://doaj.org/article/00892e5561a44abd8aa87157ddee77bd

Expression of Aspergillus nidulans phy Gene in Nicotiana benthamiana Produces Active Phytase with Broad Specificities

Autor: Kim Seong Dae, Tae-Kyun Oh, Dong Kee Jeong, Nagarajan Vinod, Kyung Min Jang, Chang Won Choi, Oh Sung, Sei Chang Kim, Jae Sung Park, Rajangam Udayakumar, Suk-Min Ko

Publikováno v: International Journal of Molecular Sciences, Vol 15, Iss 9, Pp 15571-15591 (2014)
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(15): 9
International Journal of Molecular Sciences; Volume 15; Issue 9; Pages: 15571-15591
International Journal of Molecular Sciences

A full-length phytase gene (phy) of Aspergillus nidulans was amplified from the cDNA library by polymerase chain reaction (PCR), and it was introduced into a bacterial expression vector, pET-28a. The recombinant protein (rPhy-E, 56 kDa) was overexpre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::723a0f9f4d2cbf2456e92541387d7778
http://www.mdpi.com/1422-0067/15/9/15571

Chemically induced Salmonella enteritidis ghosts as a novel vaccine candidate against virulent challenge in a rat model

Autor: Kim Seong Dae, Nagarajan Vinod, Chang Won Choi, Sei Chang Kim, Oh Sung, Cheong Hwan Jung

Publikováno v: Vaccine. 32:3249-3255

Salmonella enteritidis ghosts (SEGs), non-living empty bacterial cell envelopes were generated by using the minimum inhibitory concentration (MIC) of sodium hydroxide (NaOH) and investigated as a vaccine candidate in rats. To determine the immunogeni

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb5ad5efa6bcc08aadca9bb6e53861fe
https://doi.org/10.1016/j.vaccine.2014.03.090

Characterization of Chemically-Induced Bacterial Ghosts (BGs) Using Sodium Hydroxide-Induced Vibrio parahaemolyticus Ghosts (VPGs)

Autor: Sei Chang Kim, Ki-Sung Lee, Su Hyeong Lee, Nagarajan Vinod, Hyun Jung Park, Jung Mo Koo, Han Byul Noh, Chang Won Choi, Oh Sung, Seongmi Ji

Acellular bacterial ghosts (BGs) are empty non-living bacterial cell envelopes, commonly generated by controlled expression of the cloned lysis gene E of bacteriophage PhiX174. In this study, Vibrio parahaemolyticus ghosts (VPGs) were generated by ch

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::41d547d04d031c823e0e592cb7ee3708
https://doi.org/10.20944/preprints201608.0162.v1

Antioxidant Effect of Dietary Supplement Withania somnifera L. Reduce Blood Glucose Levels in Alloxan-Induced Diabetic Rats

Autor: A. Vasudevan, Sei Chang Kim, Chang Won Choi, Thankaraj Salammal Mariashibu, S. Kasthurirengan, Andy Ganapathi, Jesudass Joseph Sahaya Rayan, Rajangam Udayakumar

Publikováno v: Plant Foods for Human Nutrition. 65:91-98

The phenolic compounds and flavonoids were determined from the extracts of Withania somnifera root (WSREt) and leaf (WSLEt). The WSREt has 28.26 mg/g total phenolic compounds and 17.32 mg/g flavonoids, whereas WSLEt has 5.4 mg/g total phenolic compou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5acf9997279d532ffa0c91e03a059ab6
https://doi.org/10.1007/s11130-009-0146-8