Synthetic repertoires derived from convalescent COVID-19 patients enable discovery of SARS-CoV-2 neutralizing antibodies and a novel quaternary binding modality.

Autor: Goike J; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA., Hsieh CL; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA., Horton A; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA., Gardner EC; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA., Bartzoka F; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA., Wang N; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA., Javanmardi K; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA., Herbert A; U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD, USA., Abbassi S; U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD, USA., Renberg R; CCDC Army Research Laboratory, Biotechnology Branch, Adelphi, MD, USA., Johanson MJ; National Center for Therapeutics Manufacturing, Texas A&M University, College Station, TX, USA., Cardona JA; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA., Segall-Shapiro T; CCDC Army Research Laboratory-South, Austin, TX, USA., Zhou L; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA., Nissly RH; Department of Veterinary and Biomedical Science and Animal Diagnostic Laboratory, The Pennsylvania State University, University Park, Pennsylvania, USA., Gontu A; Department of Veterinary and Biomedical Science and Animal Diagnostic Laboratory, The Pennsylvania State University, University Park, Pennsylvania, USA., Byrom M; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA., Maranhao AC; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA., Battenhouse AM; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA., Gejji V; National Center for Therapeutics Manufacturing, Texas A&M University, College Station, TX, USA., Soto-Sierra L; National Center for Therapeutics Manufacturing, Texas A&M University, College Station, TX, USA.; Department of Agricultural and Biological Engineering, Texas A&M University, College Station, TX, USA., Foster ER; National Center for Therapeutics Manufacturing, Texas A&M University, College Station, TX, USA.; Department of Agricultural and Biological Engineering, Texas A&M University, College Station, TX, USA., Woodard SL; National Center for Therapeutics Manufacturing, Texas A&M University, College Station, TX, USA., Nikolov ZL; National Center for Therapeutics Manufacturing, Texas A&M University, College Station, TX, USA.; Department of Agricultural and Biological Engineering, Texas A&M University, College Station, TX, USA., Lavinder J; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA., Voss WN; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA., Annapareddy A; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; CCDC Army Research Laboratory-South, Austin, TX, USA., Ippolito GC; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Oncology, Dell Medical School, The University of Texas at Austin, Austin, TX, USA., Ellington AD; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Chemistry, The University of Texas at Austin, Austin, TX, USA., Marcotte EM; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA., Finkelstein IJ; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA., Hughes RA; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; CCDC Army Research Laboratory-South, Austin, TX, USA., Musser JM; Center for Molecular and Translational Human Infectious Diseases Research, Department of Pathology and Genomic Medicine, Houston Methodist Research Institute, Houston Methodist Hospital, Houston, TX, USA., Kuchipudi SV; Department of Veterinary and Biomedical Science and Animal Diagnostic Laboratory, The Pennsylvania State University, University Park, Pennsylvania, USA., Kapur V; Department of Animal Science and the Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, Pennsylvania, USA., Georgiou G; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, USA.; Department of Chemistry, The University of Texas at Austin, Austin, TX, USA.; Department of Oncology, Dell Medical School, The University of Texas at Austin, Austin, TX, USA., Dye JM; U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD, USA., Boutz DR; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; CCDC Army Research Laboratory-South, Austin, TX, USA., McLellan JS; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA., Gollihar JD; Center for Systems and Synthetic Biology, Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.; CCDC Army Research Laboratory-South, Austin, TX, USA.; Center for Molecular and Translational Human Infectious Diseases Research, Department of Pathology and Genomic Medicine, Houston Methodist Research Institute, Houston Methodist Hospital, Houston, TX, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2021 Apr 09. Date of Electronic Publication: 2021 Apr 09.