Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Sebastian N. Kieper"'
Autor:
Sebastian N. Kieper, Cristóbal Almendros, Juliane Behler, Rebecca E. McKenzie, Franklin L. Nobrega, Anna C. Haagsma, Jochem N.A. Vink, Wolfgang R. Hess, Stan J.J. Brouns
Publikováno v:
Cell Reports, Vol 22, Iss 13, Pp 3377-3384 (2018)
Summary: CRISPR-Cas systems adapt their immunological memory against their invaders by integrating short DNA fragments into clustered regularly interspaced short palindromic repeat (CRISPR) loci. While Cas1 and Cas2 make up the core machinery of the
Externí odkaz:
https://doaj.org/article/a553cb692f094045896dfc2de824af60
Autor:
Cristóbal Almendros, Albert J. R. Heck, Arjan Barendregt, Anna C. Haagsma, Stan J. J. Brouns, Sebastian N. Kieper
Publikováno v:
CRISPR Journal, 4(4)
The immunization of bacteria and archaea against invading viruses via CRISPR adaptation is critically reliant on the efficient capture, accurate processing, and integration of CRISPR spacers into the host genome. The adaptation proteins Cas1 and Cas2
Publikováno v:
FEMS Microbiology Letters
FEMS Microbiology Letters 366 (2019) 11
FEMS Microbiology Letters, 366(11)
FEMS Microbiology Letters 366 (2019) 11
FEMS Microbiology Letters, 366(11)
Integrating short DNA fragments at the correct leader-repeat junction is key to successful CRISPR-Cas memory formation. The Cas1–2 proteins are responsible to carry out this process. However, the CRISPR adaptation process additionally requires a DN
Autor:
Cristóbal Almendros, Jochem N.A. Vink, Juliane Behler, Rebecca E. McKenzie, Anna C. Haagsma, Stan J. J. Brouns, Sebastian N. Kieper, Wolfgang R. Hess, Franklin L. Nobrega
Publikováno v:
Cell Reports 22 (2018) 13
Cell Reports, 22(13)
Cell Reports, 22(13), 3377-3384
Cell Reports
Cell Reports, Vol 22, Iss 13, Pp 3377-3384 (2018)
Cell Reports, 22(13)
Cell Reports, 22(13), 3377-3384
Cell Reports
Cell Reports, Vol 22, Iss 13, Pp 3377-3384 (2018)
Summary CRISPR-Cas systems adapt their immunological memory against their invaders by integrating short DNA fragments into clustered regularly interspaced short palindromic repeat (CRISPR) loci. While Cas1 and Cas2 make up the core machinery of the C
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d0fd6978c54e9f52133ab25b786a07f
https://research.wur.nl/en/publications/cas4-facilitates-pam-compatible-spacer-selection-during-crispr-ad
https://research.wur.nl/en/publications/cas4-facilitates-pam-compatible-spacer-selection-during-crispr-ad
Publikováno v:
Molecular Cell, 73(4), 641-642
Molecular Cell 73 (2019) 4
Molecular Cell 73 (2019) 4
CRISPR-Cas9 genome editing has transformed biotechnology and therapeutics. However, in vivo applications of some Cas9s are hindered by large size [limiting delivery by adeno-associated virus (AAV) vectors], off-target editing, or complex protospacer
Autor:
Robert D. Fagerlund, Stan J. J. Brouns, Peter C. Fineran, Sebastian N. Kieper, Rebecca E. McKenzie, Simon A. Jackson
Publikováno v:
Science, 356(6333)
Science
Science 356 (2017) 6333
Science
Science 356 (2017) 6333
Variation in prokaryote adaptive immunity To repel infection by phage and mobile genetic elements, prokaryotes have a form of adaptive immune response and memory invested in clustered regularly interspaced short palindromic repeats and associated pro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4cdac42523dbb8e5f53d436dc4760fb5
http://resolver.tudelft.nl/uuid:0baaa190-8e07-4c91-9114-1bd0cc430238
http://resolver.tudelft.nl/uuid:0baaa190-8e07-4c91-9114-1bd0cc430238
Autor:
Willem R Miellet, Tim Künne, Stan J. J. Brouns, Anne Ilse Maria Vogel, Misha Klein, Maria Suarez-Diez, Martin Depken, Jasper W. Bannenberg, Sebastian N. Kieper
Publikováno v:
Molecular Cell
Molecular Cell, 63(5), 852-864
Molecular Cell, 63(5)
Molecular Cell 63 (2016) 5
Molecular Cell, 63(5), 852-864
Molecular Cell, 63(5)
Molecular Cell 63 (2016) 5
Prokaryotes use a mechanism called priming to update their CRISPR immunological memory to rapidly counter revisiting, mutated viruses, and plasmids. Here we have determined how new spacers are produced and selected for integration into the CRISPR arr
Autor:
Peter C. Fineran, Helen K. Opel-Reading, Raymond H.J. Staals, Max E. Wilkinson, Sebastian N. Kieper, Rebecca E. McKenzie, Yoshio Nakatani, Kurt L. Krause
Publikováno v:
Biochemical Journal 473 (2016) 8
Biochemical Journal, 473(8), 1063-1072
Biochemical Journal, 473(8), 1063-1072
CRISPR–Cas systems are adaptive immune systems in prokaryotes that provide protection against viruses and other foreign DNA. In the adaptation stage, foreign DNA is integrated into CRISPR (clustered regularly interspaced short palindromic repeat) a