Critical redundant functions of the adapters Grb2 and Gads in platelet (hem)ITAM signaling in mice

Autor: Timo Vögtle, Ayesha A. Baig, Julia Volz, Timothy B. Duchow, Irina Pleines, Sebastian Dütting, Lars Nitschke, Stephen P. Watson, Bernhard Nieswandt

Publikováno v: Platelets, Vol 31, Iss 6, Pp 801-811 (2020)

Platelets are essential for normal hemostasis; however, pathological conditions can also trigger unwanted platelet activation precipitating thrombosis and ischemic damage of vital organs such as the heart or brain. Glycoprotein (GP)VI- and C-type lec

Externí odkaz: https://doaj.org/article/b84ba99bcc4f4a65985d1c482889fe84

A Cdc42/RhoA regulatory circuit downstream of glycoprotein Ib guides transendothelial platelet biogenesis

Autor: Sebastian Dütting, Frederique Gaits-Iacovoni, David Stegner, Michael Popp, Adrien Antkowiak, Judith M.M. van Eeuwijk, Paquita Nurden, Simon Stritt, Tobias Heib, Katja Aurbach, Oguzhan Angay, Deya Cherpokova, Niels Heinz, Ayesha A. Baig, Maximilian G. Gorelashvili, Frank Gerner, Katrin G. Heinze, Jerry Ware, Georg Krohne, Zaverio M. Ruggeri, Alan T. Nurden, Harald Schulze, Ute Modlich, Irina Pleines, Cord Brakebusch, Bernhard Nieswandt

Publikováno v: Nature Communications, Vol 8, Iss 1, Pp 1-13 (2017)

Platelets derive from large precursor cells (megakaryocytes) in the bone marrow. Düttinget al. show that megakaryocyte polarization and platelet biogenesis in the bone-marrow sinusoids are directed by adhesion receptor GPIb signalling and resulting

Externí odkaz: https://doaj.org/article/9a41c7db2ef649429ea7c2db2ddf72fe

Impaired microtubule dynamics contribute to microthrombocytopenia in RhoB-deficient mice

Autor: Maximilian Englert, Katja Aurbach, Isabelle C. Becker, Annika Gerber, Tobias Heib, Lou M. Wackerbarth, Charly Kusch, Kristina Mott, Gabriel H. M. Araujo, Ayesha A. Baig, Sebastian Dütting, Ulla G. Knaus, Christian Stigloher, Harald Schulze, Bernhard Nieswandt, Irina Pleines, Zoltan Nagy

Publikováno v: Blood advances. 6(17)

Megakaryocytes are large cells in the bone marrow that give rise to blood platelets. Platelet biogenesis involves megakaryocyte maturation, the localization of the mature cells in close proximity to bone marrow sinusoids, and the formation of protrus

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d5561274d89202a19d9111fe8bad368
https://pubmed.ncbi.nlm.nih.gov/35819450

RhoA/Cdc42 signaling drives cytoplasmic maturation but not endomitosis in megakaryocytes

Autor: Annika Gerber, Isabelle C. Becker, Heike M. Hermanns, Philipp Burkard, Mara Meub, Bernhard Nieswandt, Charly Kusch, Deya Cherpokova, Daniel Jahn, Harald Schulze, Cord Brakebusch, Tobias Heib, Georgi Manukjan, Zoltan Nagy, Maximilian Englert, Thomas Dandekar, Irina Pleines, Christian Stigloher, Johannes Balkenhol, Sarah Beck, Lou Martha Wackerbarth, Sebastian Dütting, Markus Sauer

Publikováno v: Heib, T, Hermanns, H M, Manukjan, G, Englert, M, Kusch, C, Becker, I C, Gerber, A, Wackerbarth, L M, Burkard, P, Dandekar, T, Balkenhol, J, Jahn, D, Beck, S, Meub, M, Dütting, S, Stigloher, C, Sauer, M, Cherpokova, D, Schulze, H, Brakebusch, C, Nieswandt, B, Nagy, Z & Pleines, I 2021, ' RhoA/Cdc42 signaling drives cytoplasmic maturation but not endomitosis in megakaryocytes ', Cell Reports, vol. 35, no. 6, pp. 109102 . https://doi.org/10.1016/j.celrep.2021.109102

Summary Megakaryocytes (MKs), the precursors of blood platelets, are large, polyploid cells residing mainly in the bone marrow. We have previously shown that balanced signaling of the Rho GTPases RhoA and Cdc42 is critical for correct MK localization

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24b4b43aea7a435a9277628b412b7b5e
https://curis.ku.dk/ws/files/271679450/1_s2.0_S2211124721004368_main.pdf

A defined metabolic state in pre B cells governs B-cell development and is counterbalanced by Swiprosin-2/EFhd1

Autor: Dirk Mielenz, Tobit Steinmetz, Kristin Fritsch, Merle Stein, Hans-Martin Jäck, Sophia Urbanczyk, Michael R. Bösl, Thomas Winkler, Wolfgang Schuh, Sebastian Dütting, Dimitrios Mougiakakos, Aline Bozec, Dorothea Reimer

B-cell development in the bone marrow comprises proliferative and resting phases in different niches. We asked whether B-cell metabolism relates to these changes. Compared to pro B and small pre B cells, large pre B cells revealed the highest glucose

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87bf639c2b2b107a7fa68e05d6e4926c
https://europepmc.org/articles/PMC5520169/