Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Sean P. Leonard"'
Autor:
Noor Radde, Genevieve A. Mortensen, Diya Bhat, Shireen Shah, Joseph J. Clements, Sean P. Leonard, Matthew J. McGuffie, Dennis M. Mishler, Jeffrey E. Barrick
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-17 (2024)
Abstract Engineered DNA will slow the growth of a host cell if it redirects limiting resources or otherwise interferes with homeostasis. Escape mutants that alleviate this burden can rapidly evolve and take over cell populations, making genetic engin
Externí odkaz:
https://doaj.org/article/d2a482698d444693acde04b2a15a0850
Autor:
Patrick J. Lariviere, A. H. M. Zuberi Ashraf, Lucio Navarro-Escalante, Sean P. Leonard, Laurel G. Miller, Nancy A. Moran, Jeffrey E. Barrick
Publikováno v:
mBio, Vol 15, Iss 9 (2024)
ABSTRACT Mechanistic understanding of interactions in many host-microbe systems, including the honey bee microbiome, is limited by a lack of easy-to-use genome engineering approaches. To this end, we demonstrate a one-step genome engineering approach
Externí odkaz:
https://doaj.org/article/e01b36d64072430397920f0252eda892
Publikováno v:
Microbiology Spectrum, Vol 9, Iss 1 (2021)
ABSTRACT Antibiotics have been applied to honey bee (Apis mellifera) hives for decades to treat Paenibacillus larvae, which causes American foulbrood disease and kills honey bee larvae. One of the few antibiotics approved in apiculture is tylosin tar
Externí odkaz:
https://doaj.org/article/4ac634a028774b5f837b421af51873ca
Autor:
Patrick J. Lariviere, Sean P. Leonard, Richard D. Horak, J. Elijah Powell, Jeffrey E. Barrick
Publikováno v:
Nature Protocols. 18:902-928
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9278d490c8e69d88c812d2413791ccbb
https://doi.org/10.1038/s41596-022-00778-4
https://doi.org/10.1038/s41596-022-00778-4
Autor:
Jennifer L. Chlebek, Sean P. Leonard, Christina Kang-Yun, Mimi C. Yung, Dante P. Ricci, Yongqin Jiao, Dan M. Park
The development of synthetic biological circuits that maintain functionality over application relevant timescales remains a significant challenge. Here, we employed synthetic overlapping sequences in which one gene is encoded or “entangled” entir
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::65cf2f045080e1ad41be81f2c24dbc33
https://doi.org/10.1101/2023.02.27.530340
https://doi.org/10.1101/2023.02.27.530340
Autor:
Katherine M. Elston, Laila E. Phillips, Sean P. Leonard, Eleanor Young, Jo-anne C. Holley, Tasneem Ahsanullah, Braydin McReynolds, Nancy A. Moran, Jeffrey E. Barrick
Publikováno v:
bioRxiv
Toolkits of plasmids and genetic parts streamline the process of assembling DNA constructs and engineering microbes. Many of these kits were designed with specific industrial or laboratory microbes in mind. For researchers interested in non-model mic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2160fd02f0d00d24140fe2f5ec92281
https://europepmc.org/articles/PMC9949093/
https://europepmc.org/articles/PMC9949093/
Publikováno v:
Philosophical transactions of the Royal Society of London. Series B, Biological sciences. 377(1853)
Social corbiculate bees are major pollinators. They have characteristic bacterial microbiomes associated with their hives and their guts. In honeybees and bumblebees, worker guts contain a microbiome composed of distinctive bacterial taxa shown to be
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0fa7eacbe914ec3ed619f1302eca81a
https://pubmed.ncbi.nlm.nih.gov/35491599
https://pubmed.ncbi.nlm.nih.gov/35491599
Autor:
Jiri Perutka, Peng Geng, Andrew D. Ellington, Jeffrey E. Barrick, Bryan William Davies, J. Elijah Powell, Sean P. Leonard, Richard D. Horak, Nancy A. Moran, Luke C. Heckmann
Publikováno v:
Science. 367:573-576
Inducing immune bee genes Honey bees are prone to parasitism by the Varroa mite, which is a vector for several bee pathogens. However, honey bees are also host to the symbiotic gut bacterium Snodgrassella alvi. Leonard et al. engineered S. alvi to pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b92a3a58cb27d3c73ac4aecb9de6ece
https://doi.org/10.1126/science.aax9039
https://doi.org/10.1126/science.aax9039
Publikováno v:
Microbiology Spectrum, Vol 9, Iss 1 (2021)
Microbiology Spectrum
Microbiology Spectrum
Antibiotics have been applied to honey bee (Apis mellifera) hives for decades to treat Paenibacillus larvae, which causes American foulbrood disease and kills honey bee larvae. One of the few antibiotics approved in apiculture is tylosin tartrate. Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c80b08b445151a2e58853f4b65a1818
https://journals.asm.org/doi/10.1128/Spectrum.00103-21
https://journals.asm.org/doi/10.1128/Spectrum.00103-21
Publikováno v:
ACS Synthetic Biology. 8:521-531
Mobile genetic elements drive evolution by disrupting genes and rearranging genomes. Eukaryotes have evolved epigenetic mechanisms, including DNA methylation and RNA interference, that silence mobile elements and thereby preserve the integrity of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30f88b4eb2881610e7e04b52fb59509e
https://doi.org/10.1021/acssynbio.8b00426
https://doi.org/10.1021/acssynbio.8b00426