Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria

Autor: Vivek K. Mutalik, Pavel S. Novichkov, Morgan N. Price, Trenton K. Owens, Mark Callaghan, Sean Carim, Adam M. Deutschbauer, Adam P. Arkin

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019)

Gain of function methods based on gene overexpression are not easily applied to high-throughput screening across different experimental conditions. Here, the authors present Dub-seq, which separates overexpression library characterization from functi

Externí odkaz: https://doaj.org/article/3444650c24824da0912c4a2da5a186b7

Systematic discovery of pseudomonad genetic factors involved in sensitivity to tailocins

Autor: Sean Carim, Alexey E. Kazakov, Vivek K. Mutalik, Morgan N. Price, Adam P. Arkin, Ashley L. Azadeh, Romy Chakraborty, Peter J. Walian, Torben Nielsen, Lauren M. Lui, Adam M. Deutschbauer

Publikováno v: The ISME journal, vol 15, iss 8
The ISME Journal

Tailocins are bactericidal protein complexes produced by a wide variety of bacteria to compete against closely related strains. Like tailed bacteriophages, with whom they share an evolutionary and morphological relationship, tailocins bind and kill a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d23c1674ef0c326b047be54fd4607bcc
https://escholarship.org/uc/item/9wd3p4sh

Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria

Autor: Mark Callaghan, Vivek K. Mutalik, Pavel S. Novichkov, Trenton K. Owens, Adam P. Arkin, Sean Carim, Morgan N. Price, Adam M. Deutschbauer

Publikováno v: Nature Communications
Mutalik, Vivek K; Novichkov, Pavel S; Price, Morgan N; Owens, Trenton K; Callaghan, Mark; Carim, Sean; et al.(2019). Dual-barcoded shotgun expression library sequencing for high-throughput characterization of functional traits in bacteria. NATURE COMMUNICATIONS, 10(1), 308. doi: 10.1038/s41467-018-08177-8. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/2dv810fx
Nature communications, vol 10, iss 1
Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019)

A major challenge in genomics is the knowledge gap between sequence and its encoded function. Gain-of-function methods based on gene overexpression are attractive avenues for phenotype-based functional screens, but are not easily applied in high-thro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31126a7acdc61a2e48f924a775a92e93
https://pubmed.ncbi.nlm.nih.gov/30659179

An ingestible bacterial-electronic system to monitor gastrointestinal health

Autor: Vladimir Bulovic, Joy Collins, Alison Hayward, Shane McDonnell, Phillip Nadeau, Robert Langer, Giovanni Traverso, Sean Carim, Richard Swartwout, Anantha P. Chandrakasan, Mark Mimee, Timothy K. Lu, Logan Jerger, Sarah E. Flanagan, Robert J. Citorik

Publikováno v: PMC

Biomolecular monitoring in the gastrointestinal tract could offer rapid, precise disease detection and management but is impeded by access to the remote and complex environment. Here, we present an ingestible micro-bio-electronic device (IMBED) for i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa6436fea8faf23d69708c312e715317
https://hdl.handle.net/1721.1/128441