Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Sean C. Taylor"'
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-12 (2022)
Abstract Western blotting experiments were initially performed to detect a target protein in a complex biological sample and more recently, to measure relative protein abundance. Chemiluminescence coupled with film-based detection was traditionally t
Externí odkaz:
https://doaj.org/article/5eb803a0e2b9413991b9075c7b1620f9
Autor:
Sean C. Taylor
Publikováno v:
Journal of Clinical Virology Plus, Vol 1, Iss 4, Pp 100044- (2021)
As countries globally are in the process of planning, introducing or implementing mass vaccination strategies while continuing to deal with the ongoing SARS-CoV-2 pandemic, an evolution in testing strategies may be required to minimize spread in mixe
Externí odkaz:
https://doaj.org/article/38feb318b99143a4ab2a65a5f80249b3
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
Abstract Quantitative PCR (qPCR) has become the gold standard technique to measure cDNA and gDNA levels but the resulting data can be highly variable, artifactual and non-reproducible without appropriate verification and validation of both samples an
Externí odkaz:
https://doaj.org/article/4d07ff82020847f8b55f25f42876f2de
Autor:
Marvin S. Hausman, Jeffrey M. Schapiro, Beth Hurst, Sean C. Taylor, Alexander Lituev, Ian Martiszus, Samar Sarwat, Sarah Rowell
Publikováno v:
Vaccine
The question associated with efficacy and longevity of SARS-CoV-2 protection post-vaccination is paramount. The cPass surrogate virus neutralization test (sVNT) has gained popularity globally as a dual application assay for: 1. Accurate SARS-CoV-2 po
Autor:
Ashley Bailey, Jayne Fenton, Wendy I Sligl, Sean C. Taylor, Carmen L. Charlton, Sabrina Plitt, Rafael Rivera, Sean H. Ling, Jamil N. Kanji, LeeAnn Turnbull, Graham Tipples
PURPOSEWith rapid approval of SARS-CoV-2 vaccines, the ability of clinical laboratories to detect vaccine-induced antibodies with available high-throughput commercial assays is unknown. We aimed to determine if commercial serology assays can detect v
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::27ee0eff021e50cd32917dcd17a4d173
https://doi.org/10.1101/2021.03.30.21254604
https://doi.org/10.1101/2021.03.30.21254604
Publikováno v:
Trends in Biotechnology. 37:761-774
Quantitative PCR (qPCR) is one of the most common techniques for quantification of nucleic acid molecules in biological and environmental samples. Although the methodology is perceived to be relatively simple, there are a number of steps and reagents
Autor:
Sabrina Plitt, Wendy I Sligl, Jamil N. Kanji, Graham Tipples, LeeAnn Turnbull, Sean H. Ling, Carmen L. Charlton, Sean C. Taylor, Jayne Fenton, Ashley Bailey, Rafael Rivera
Publikováno v:
Vaccine
BACKGROUND: With rapid approval of SARS-CoV-2 vaccines, the ability of clinical laboratories to detect vaccine-induced antibodies with available high-throughput commercial assays is unknown. We aimed to determine if commercial serology assays can det
Autor:
Sean C. Taylor, Kyle Annen, Beth Hurst, Vijaya Knight, Thomas E. Morrison, Melkon G DomBourian, Ashley Bailey, Jamil N. Kanji, Carmen L. Charlton, Leah Huey, Mary K. McCarthy
Publikováno v:
Journal of Clinical Microbiology
Many severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serology tests have proven to be less accurate than expected and do not assess antibody function as neutralizing, correlating with protection from reinfection. A new assay technology m
Publikováno v:
Molecular Biotechnology
Chemiluminescent western blotting has been in common practice for over three decades, but its use as a quantitative method for measuring the relative expression of the target proteins is still debatable. This is mainly due to the various steps, techn
Publikováno v:
Journal of virological methods. 224
The recent introduction of Droplet Digital PCR (ddPCR) has provided researchers with a tool that permits direct quantification of nucleic acids from a wide range of samples with increased precision and sensitivity versus RT-qPCR. The sample interdepe