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pro vyhledávání: '"Scott Woody"'
Publikováno v:
Journal of Molecular Biology. 240:119-126
A detailed kinetic analysis demonstrates that, in vitro , mutations in the P RM promoter of bacteriophage λ can increase the rate of open complex formation at the divergent, lytic promoter P R in either of two ways. (1) P - RM mutations, typified by
Publikováno v:
Journal of Molecular Biology. 229:37-51
We have deleted a single base-pair in the -35 region of the bacteriophage lambda PRM promoter. The deletion (PRM delta 34) creates a better match of PRM to consensus, thereby substantially increasing the activity of the promoter in vitro and in vivo.
Modulation of P(RM) activity by the lambda PR promoter in both the presence and absence of repressor
Publikováno v:
Journal of molecular biology. 232(3)
When the transcription startsites of the phage lambda promoters PRM and PR are separated by 82 bp (the wild-type spacing), mutating PR increases the rate of open complex formation at PRM at all RNA polymerase (RNAP) concentrations tested in vitro. Th
Publikováno v:
Nucleic acids research. 18(20)
Insertion of DNA containing PR, the early rightward promoter of bacteriophage lambda, is lethal to M13-derived vectors when the promoter directs transcription (using the '+' strand as template) toward the M13 origin of replication (ori). Lethality ca
Publikováno v:
Development; June 2002, Vol. 129 Issue: 12 p2797-806, 10p