Výsledky vyhledávání - "Scott T. Clarke"

Akademický článek

Detection of S-phase cell cycle progression using 5-ethynyl-2′-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2′-deoxyuridine antibodies

Autor: Suzanne B. Buck, Jolene Bradford, Kyle R. Gee, Brian J. Agnew, Scott T. Clarke, Adrian Salic

Publikováno v: BioTechniques, Vol 44, Iss 7, Pp 927-929 (2008)

The 5-bromo-2′-deoxyuridine (BrdU) labeling of cells followed by antibody staining has been the standard method for direct measurement of cells in the S-phase. Described is an improved method for the detection of S-phase cell cycle progression base

Externí odkaz: https://doaj.org/article/7287dc1ccc76495c92ad6fee0e78b743

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Calponin-homology domain mediated bending of membrane-associated actin filaments

Autor: Sayantika Ghosh, Mohan K. Balasubramanian, Scott T. Clarke, Darius Vasco Köster, Esther Ivorra-Molla, Saravanan Palani, Andrejus Suchenko

Publikováno v: eLife
eLife, Vol 10 (2021)

Actin filaments are central to numerous biological processes in all domains of life. Driven by the interplay with molecular motors, actin binding and actin modulating proteins, the actin cytoskeleton exhibits a variety of geometries. This includes st

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a5c4f3e7b1d5cfd590272c94210ff12
https://pubmed.ncbi.nlm.nih.gov/34269679

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Cell-Based High Content Analysis of Cell Proliferation and Apoptosis

Autor: Bhaskar S. Mandavilli, Michelle Yan, Scott T. Clarke

Publikováno v: Methods in Molecular Biology ISBN: 9781493973552

High content imaging-based cell cycle analysis allows multiplexing of various parameters including DNA content, DNA synthesis, cell proliferation, and other cell cycle markers such as phosho-histone H3. 5'-Ethynyl-2'-deoxyuridine (EdU) incorporation

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c5f59d003366dd183672f890f1870192
https://doi.org/10.1007/978-1-4939-7357-6_4

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Click Chemistry for Analysis of Cell Proliferation in Flow Cytometry

Autor: Jolene Bradford, Veronica Calderon, Scott T. Clarke

Publikováno v: Current Protocols in Cytometry

The measurement of cellular proliferation is fundamental to the assessment of cellular health, genotoxicity, and the evaluation of drug efficacy. Labeling, detection, and quantification of cells in the synthesis phase of cell cycle progression are no

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8dd2f911d58b941ca18b68f3455d3525
https://pubmed.ncbi.nlm.nih.gov/28967990

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Abstract 2438: Rapid fluorescence based assessment of RNA integrity quality using machine learning

Autor: Kathy Free, Debra Gale, Scott T. Clarke, Dylan Poulsen, Chris Vonnegut

Publikováno v: Cancer Research. 79:2438-2438

Much attention has been focused on RNA transcript bias. The quality of isolated mRNA used for RNA-seq and other downstream analyses can contribute to sample specific bias of transcript abundance. If the library is prepared from polyA enrichment of de

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::24f50a2a120adaa39a3c5e2444952e2b
https://doi.org/10.1158/1538-7445.am2019-2438

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Abstract 2734: Click colorimetric EdU proliferation and TUNEL assay: Conversion of click chemistry from fluorescent to colorimetric for use with bright field microscopy

Autor: Ben Armstrong, Fernada M. Bosada, Michael O’Grady, Carmen Finnessy, Scott T. Clarke

Publikováno v: Cancer Research. 76:2734-2734

We present here the conversion of click chemistry fluorescence-based EdU proliferation assay and TUNEL apoptosis assay to use with bright field microscopy. Advantages of having a colorimetric signal are the retention of the click chemistry work flow

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::99d745260f0b35a04244837d987b9976
https://doi.org/10.1158/1538-7445.am2016-2734

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Abstract 2169: High content screening in MCF7 and MCF10A cells show differential responses depending on oxygen levels and mechanistic readout for viability

Autor: Anderson April, Leticia A. Montoya, Kathy Kihn, Michael O’Grady, Michelle Yan, Veronica Calderon, Carmen Finnessy, Quentin Low, Marcy Wickett, Scott T. Clarke, Carolyn DeMarco

Publikováno v: Cancer Research. 76:2169-2169

Oxygen levels in typical cell culture conditions do not accurately reflect the oxygen levels cells are exposed to within the body. Furthermore, oxygen levels can vary within the tumor microenvironment. These variances can affect how cells respond to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::89551dea18480f4eaa62c5e947b97897
https://doi.org/10.1158/1538-7445.am2016-2169

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Abstract 3643: Flow cytometry multiplexing used to analyze metabolic activity and assess pharmaceutical compound toxicity as a high throughput screening tool

Autor: April Anderson, Carmen Finnessy, Veronica Calderon, Michelle Yan, Quentin Low, Scott T. Clarke, Marcy Wickett, Leticia A. Montoya, Carolyn DeMarco, Kathy Kihn, Mike O’Grady

Publikováno v: Cancer Research. 76:3643-3643

High throughput screening (HTS) is an extremely effective method for allowing researchers to identify putative active compounds for therapeutics. Recently, flow cytometry has emerged as a powerful HTS tool with the added benefit of cell-by-cell analy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2bcef3f00cd432801e70b7fed3f1dd17
https://doi.org/10.1158/1538-7445.am2016-3643

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