Zobrazeno 1 - 2
of 2
pro vyhledávání: '"Scott M. Pedler"'
Publikováno v:
Yeast. 13:119-125
Intracellular metabolic flux has been investigated in two strains of Saccharomyces cerevisiae grown into stationary phase under both glucose-repressed and glucose-derepressed conditions. By employing a variety of simple methodologies (manometry, enzy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f217fba0a98eb32408da94a9f70a5de3
https://doi.org/10.1002/(sici)1097-0061(199702)13:2<119::aid-yea67>3.0.co
https://doi.org/10.1002/(sici)1097-0061(199702)13:2<119::aid-yea67>3.0.co
Publikováno v:
Analytical biochemistry. 222(2)
A method is described for rapidly quenching metabolism in yeast and extracting metabolites for analysis. The saponin digitonin is used to permeabilize the yeast cell membrane, in conjunction with perchloric acid (PCA) to quench metabolism and extract
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca6a9c94b98fbd4c2245491af8918c37
https://pubmed.ncbi.nlm.nih.gov/7864365
https://pubmed.ncbi.nlm.nih.gov/7864365