Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Scott Frykman"'
Publikováno v:
Journal of Industrial Microbiology & Biotechnology. 33:445-453
Various bioactive small molecules produced by microbial cultivation are degraded in the culture broth or may repress the formation of additional product. The inclusion of hydrophobic adsorber resin beads to capture these products in situ and remove t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::492f173b64df2d2ebb73019f1fd3e407
https://doi.org/10.1007/s10295-006-0088-1
https://doi.org/10.1007/s10295-006-0088-1
Publikováno v:
Biotechnology Progress. 21:1102-1108
Epothilone D is a member of a class of potent antineoplastic natural products produced by myxobacteria. Previously, we have described a fed-batch epothilone D production process in which an adsorber resin is incorporated into the bioreactor setup to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a5254dec9f86470b70adab0d1fb85b71
https://doi.org/10.1021/bp050010+
https://doi.org/10.1021/bp050010+
Autor:
Sajel Patel, Sally Ou, Christopher Carreras, Peter J. Licari, Timothy Leaf, Scott Frykman, Lawrence Cadapan, Elaine Woo, Gary W. Ashley, John R. Carney, Mark A. Burlingame, Stefan Zavala
Publikováno v:
Journal of Biotechnology. 92:217-228
A method was developed for the large-scale bioconversion of novel 6-deoxyerythronolide B (6-dEB) analogs into erythromycin analogs. Erythromycin biosynthesis in Saccharopolyspora erythraea proceeds via the formation of a polyketide aglycone, 6-dEB, w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b8afea1ad008ceaa79b68c8059b8f16
https://doi.org/10.1016/s0168-1656(01)00372-8
https://doi.org/10.1016/s0168-1656(01)00372-8
Autor:
Janice Lau, Rika Regentin, Christopher D. Reeves, Daniel V. Santi, Scott Frykman, Hiroko Tsuruta, Sally Ou, Peter J. Licari, John R. Carney
Publikováno v:
Journal of Industrial Microbiology & Biotechnology. 28:17-20
Recently, the epothilone polyketide synthase (PKS) was successfully introduced into a heterologous production host for the large-scale production of epothilone D. We have found that at least three other epothilones can also be produced as the major f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::031f21a259d7ff834fa6199b5f106950
https://doi.org/10.1038/sj.jim.7000209
https://doi.org/10.1038/sj.jim.7000209
Publikováno v:
Biotechnology and Bioengineering. 76:303-310
Diketide N-acetylcysteamine (diketide NAC) thioester precursors were fed to 6-Deoxyerythronolide B synthase (DEBS) ketosynthase-1 inactivated (KS1 degree) Saccharopolyspora erythraea strains to produce 13-substituted erythromycin analogs. This direct
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd8ba20ff8ce3d6527ad71c9d80b795d
https://doi.org/10.1002/bit.10086
https://doi.org/10.1002/bit.10086
Autor:
Friedrich Srienc, Scott Frykman
Publikováno v:
Biotechnology and Bioengineering. 76:259-268
Synchronized Saccharomyces cerevisiae cell populations were used to examine secretion rates of a heterologous protein as a function of cell cycle position. The synchronization procedure had a profound effect on the type and quality of data obtained.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e075dd92b5ebcd3237f1de70991ceb8
https://doi.org/10.1002/bit.10003
https://doi.org/10.1002/bit.10003
Autor:
Scott Frykman, Friedrich Srienc
Publikováno v:
Biotechnology and Bioengineering. 59:214-226
To observe events occurring in the microenvironment surrounding individual cells, a mathematical framework has been developed describing the behavior of a compound following its secretion by a single cell. This description is based on the diffusional
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32b845a8da422cfc11ac82d9ddf24f8a
https://doi.org/10.1002/(sici)1097-0290(19980720)59:2<214::aid-bit9>3.0.co
https://doi.org/10.1002/(sici)1097-0290(19980720)59:2<214::aid-bit9>3.0.co
Publikováno v:
Biotechnology and bioengineering. 78(3)
The heterologous production of epothilone D in Myxococcus xanthus was improved by 140-fold from an initial titer of 0.16 mg/L with the incorporation of an adsorber resin, the identification of a suitable carbon source, and the implementation of a fed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3667ee82b6c593dfdb7cacd0e1655cc
https://pubmed.ncbi.nlm.nih.gov/11920444
https://pubmed.ncbi.nlm.nih.gov/11920444