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pro vyhledávání: '"Scott B. Mulrooney"'
Autor:
Scott B. Mulrooney, Crisjoe A. Joseph, Jodi L. Boer, Robert P. Hausinger, Vlad Martin-Diaconescu, Michael J. Maroney
Publikováno v:
JBIC Journal of Biological Inorganic Chemistry. 22:497-503
Nickel-dependent ureases are activated by a multiprotein complex that includes the GTPase UreG. Prior studies showed that nucleotide-free UreG from Klebsiella aerogenes is monomeric and binds one nickel or zinc ion with near-equivalent affinity using
Autor:
Melanie McNeil, Berok Asi, Yiming Shan, Scott B. Mulrooney, Monica Joya, Nandini Ravichandran, Satya Prakash, Ricardo Duque, Maryam Mobed-Miremadi
Publikováno v:
Artificial cells, nanomedicine, and biotechnology. 46(sup2)
Second generation E. coli DH5α (pKAU17) was successfully encapsulated by means of atomization (MA), inkjet printing (MI) and double-encapsulation (DDMI) for the purpose of urea degradation in a simulated uremic medium at 37 °C. Experimentally deter
Autor:
Robert P. Hausinger, Ben B. C. Ko, Scott B. Mulrooney, Hongzhe Sun, Yi-Bo Zeng, Andy F. K. Leung, Li Zhang
Publikováno v:
BioMetals. 19:503-511
Bismuth compounds are widely used for the treatment of peptic ulcers and Helicobacter pylori infections. It has been suggested that enzyme inhibition plays an important role in the antibacterial activity of bismuth towards this bacterium. Urease, an
Publikováno v:
Journal of Bacteriology. 188:223-230
Upon exposure to alkylating agents, Escherichia coli increases expression of aidB along with three genes ( ada , alkA , and alkB ) that encode DNA repair proteins. In order to begin to identify the role of AidB in the cell, the protein was purified t
Publikováno v:
Journal of Bacteriology. 187:3581-3585
Klebsiella aerogenes UreE, a metallochaperone that delivers nickel ions during urease activation, consists of distinct “peptide-binding” and “metal-binding” domains and a His-rich C terminus. Deletion analyses revealed that the metal-binding
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 1674:319-326
Cytochrome b5 (cyt b5) is an amphipathic membrane-bound heme protein found in the endoplasmic reticulum of eukaryotes. It consists of three domains, an N-terminal cytosolic, hydrophilic domain containing the heme, a short flexible linker and an alpha
Autor:
Adelbert Bacher, Charles H. Williams, Scott B. Mulrooney, Franz Müller, Kristina Kemter, Wolfgang Eisenreich
Publikováno v:
European Journal of Biochemistry. 271:1437-1452
Thioredoxin reductase (TrxR) from Escherichia coli, the mutant proteins E159Y and C138S, and the mutant protein C138S treated with phenylmercuric acetate were reconstituted with [U-(13)C(17),U-(15)N(4)]FAD and analysed, in their oxidized and reduced
Publikováno v:
Journal of Bacteriology. 185:126-134
Allantoinase is a suspected dinuclear metalloenzyme that catalyzes the hydrolytic cleavage of the five-member ring of allantoin (5-ureidohydantoin) to form allantoic acid. Recombinant Escherichia coli allantoinase purified from overproducing cultures
This chapter deals with the basic physical analytical methods and separation procedures of spectrophotometry, electrodes, chromatography, radioactivity, and electrophoresis. It talks about the simple procedures which are appropriate for laboratory cl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e9848fb0ab9b6a4343a702e581c3a87f
https://doi.org/10.1128/9781555817497.ch17
https://doi.org/10.1128/9781555817497.ch17
Autor:
Scott B. Mulrooney, Lucy Waskell
Publikováno v:
Protein Expression and Purification. 19:173-178
Expression of the membrane-bound form of rabbit cytochrome b(5) in Escherichia coli has been significantly improved through the use of the T7 expression vector pLW01 (A. Bridges, L. Gruenke, Y.-T. Chang, I. Vakser, G. Loew, and L. Waskell, 1998, J. B