Zobrazeno 1 - 5
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pro vyhledávání: '"Scott A. Zugel"'
Autor:
Scott A. Zugel, Fred E. Lytle
Publikováno v:
Applied Spectroscopy. 54:1490-1494
Two geometries of excitation/emission optics are compared for two-photon excitation of time-resolved fluorescence in capillaries. The test fluorophore is the enzymatic tag β-naphthylamine (BNA). Two 0.25 NA (numerical aperture) microscope objectives
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0cbc2948b6cc72ea813af9cf9d4cd9e3
https://doi.org/10.1366/0003702001948402
https://doi.org/10.1366/0003702001948402
Autor:
Fred E. Lytle, Scott A. Zugel
Publikováno v:
Applied Spectroscopy. 54:1203-1207
The loss in sensitivity of two-photon excited fluorescence, when switching from a 1 cm cell format to square and cylindrical capillaries, is described quantitatively. Sensitivities are determined with calibration curves, and beam shapes are computed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ca25664d79efbd8be732c9da14c97250
https://doi.org/10.1366/0003702001950779
https://doi.org/10.1366/0003702001950779
Publikováno v:
Applied Spectroscopy. 50:1335-1337
Trace-level impurities, such as those found in organic solvents and aqueous buffer solutions, are known to have little contribution to the two-photon excited fluorescence blank. Previously, the major source of interference in 90° fluorescence detect
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2218e97871a8b541cd18d1793ca95b87
https://doi.org/10.1366/0003702963905033
https://doi.org/10.1366/0003702963905033
Publikováno v:
Analytical chemistry. 72(22)
Two-photon excited fluorescence detection was performed on a microfabricated electrophoresis chip. A calibration curve of the fluorescent tag beta-naphthylamine was performed, resulting in a sensitivity of 2.5 x 10(9) counts M(-1) corresponding to a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3067c35a0bf8fbb93fb69c5a9d0af5af
https://pubmed.ncbi.nlm.nih.gov/11101255
https://pubmed.ncbi.nlm.nih.gov/11101255
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