Genetic Engineering in Combination with Semi-Synthesis Leads to a New Route for Gram-Scale Production of the Immunosuppressive Natural Product Brasilicardin A.

Autor: Botas A; Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo and Instituto de Investigación Sanitaria de Asturias (ISPA), c/ Julián Clavería s/n., 33006, Oviedo, Spain., Eitel M; Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Tübingen, Auf der Morgenstelle 8, 72076, Tübingen, Germany., Schwarz PN; Department of Microbiology and Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Auf der Morgenstelle 28, 72076, Tübingen, Germany., Buchmann A; Department of Pharmaceutical Biology, Institute of Pharmaceutical Sciences, University of Tübingen, Auf der Morgenstelle 8, 72076, Tübingen, Germany., Costales P; EntreChem S.L., Vivero Ciencias de la Salud, c/ Colegio Santo Domingo de Guzmán, s/n, 33011, Oviedo, Spain., Núñez LE; EntreChem S.L., Vivero Ciencias de la Salud, c/ Colegio Santo Domingo de Guzmán, s/n, 33011, Oviedo, Spain., Cortés J; EntreChem S.L., Vivero Ciencias de la Salud, c/ Colegio Santo Domingo de Guzmán, s/n, 33011, Oviedo, Spain., Morís F; EntreChem S.L., Vivero Ciencias de la Salud, c/ Colegio Santo Domingo de Guzmán, s/n, 33011, Oviedo, Spain., Krawiec M; Department of Molecular Microbiology, Faculty of Biotechnology, University of Wrocław, ul. F. Joliot-Curie 14A, 50-383, Wrocław, Poland., Wolański M; Department of Molecular Microbiology, Faculty of Biotechnology, University of Wrocław, ul. F. Joliot-Curie 14A, 50-383, Wrocław, Poland., Gust B; Department of Pharmaceutical Biology, Institute of Pharmaceutical Sciences, University of Tübingen, Auf der Morgenstelle 8, 72076, Tübingen, Germany., Rodriguez M; Quadriga BioSciences, Inc., 339 S. San Antonio Road, Suite 2A, Los Altos, CA, 94022, USA., Fischer WN; Quadriga BioSciences, Inc., 339 S. San Antonio Road, Suite 2A, Los Altos, CA, 94022, USA., Jandeleit B; Quadriga BioSciences, Inc., 339 S. San Antonio Road, Suite 2A, Los Altos, CA, 94022, USA., Zakrzewska-Czerwińska J; Department of Molecular Microbiology, Faculty of Biotechnology, University of Wrocław, ul. F. Joliot-Curie 14A, 50-383, Wrocław, Poland., Wohlleben W; Department of Microbiology and Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Auf der Morgenstelle 28, 72076, Tübingen, Germany., Stegmann E; Department of Microbiology and Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Auf der Morgenstelle 28, 72076, Tübingen, Germany., Koch P; Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Tübingen, Auf der Morgenstelle 8, 72076, Tübingen, Germany., Méndez C; Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo and Instituto de Investigación Sanitaria de Asturias (ISPA), c/ Julián Clavería s/n., 33006, Oviedo, Spain., Gross H; Department of Pharmaceutical Biology, Institute of Pharmaceutical Sciences, University of Tübingen, Auf der Morgenstelle 8, 72076, Tübingen, Germany.

Publikováno v: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2021 Jun 07; Vol. 60 (24), pp. 13536-13541. Date of Electronic Publication: 2021 May 01.

The Immunosuppressant Brasilicardin: Determination of the Biosynthetic Gene Cluster in the Heterologous Host Amycolatopsis japonicum.

Autor: Schwarz PN; Microbiology/Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), Eberhard Karls University of Tübingen, Tübingen, Germany., Buchmann A; Department of Pharmaceutical Biology, Pharmaceutical Institute, Eberhard Karls University of Tübingen, Tübingen, Germany., Roller L; Microbiology/Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), Eberhard Karls University of Tübingen, Tübingen, Germany., Kulik A; Microbiology/Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), Eberhard Karls University of Tübingen, Tübingen, Germany., Gross H; Department of Pharmaceutical Biology, Pharmaceutical Institute, Eberhard Karls University of Tübingen, Tübingen, Germany.; German Centre for Infection Research (DZIF), Partner Site Tübingen, Tübingen, Germany., Wohlleben W; Microbiology/Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), Eberhard Karls University of Tübingen, Tübingen, Germany.; German Centre for Infection Research (DZIF), Partner Site Tübingen, Tübingen, Germany., Stegmann E; Microbiology/Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), Eberhard Karls University of Tübingen, Tübingen, Germany.; German Centre for Infection Research (DZIF), Partner Site Tübingen, Tübingen, Germany.

Publikováno v: Biotechnology journal [Biotechnol J] 2018 Feb; Vol. 13 (2). Date of Electronic Publication: 2017 Nov 13.

High-Quality Draft Genome Sequence of the Actinobacterium Nocardia terpenica IFM 0406, Producer of the Immunosuppressant Brasilicardins, Using Illumina and PacBio Technologies.

Autor: Buchmann A; Department of Pharmaceutical Biology, Institute of Pharmaceutical Sciences, University of Tübingen, Tübingen, Germany.; German Centre for Infection Research (DZIF), Partner Site Tübingen, Tübingen, Germany., Eitel M; Department of Medicinal and Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Tübingen, Tübingen, Germany., Koch P; Department of Medicinal and Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Tübingen, Tübingen, Germany., Schwarz PN; Department of Microbiology and Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Tübingen, Germany., Stegmann E; German Centre for Infection Research (DZIF), Partner Site Tübingen, Tübingen, Germany.; Department of Microbiology and Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Tübingen, Germany., Wohlleben W; German Centre for Infection Research (DZIF), Partner Site Tübingen, Tübingen, Germany.; Department of Microbiology and Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Tübingen, Germany., Wolański M; Faculty of Biotechnology, University of Wrocław, Wrocław, Poland., Krawiec M; Faculty of Biotechnology, University of Wrocław, Wrocław, Poland., Zakrzewska-Czerwińska J; Faculty of Biotechnology, University of Wrocław, Wrocław, Poland.; Ludwik Hirszfeld Insitute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland., Méndez C; Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, Oviedo, Spain., Botas A; Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, Oviedo, Spain., Núñez LE; EntreChem S.L., Oviedo, Spain., Morís F; EntreChem S.L., Oviedo, Spain., Cortés J; EntreChem S.L., Oviedo, Spain., Gross H; Department of Pharmaceutical Biology, Institute of Pharmaceutical Sciences, University of Tübingen, Tübingen, Germany harald.gross@uni-tuebingen.de.; German Centre for Infection Research (DZIF), Partner Site Tübingen, Tübingen, Germany.

Publikováno v: Genome announcements [Genome Announc] 2016 Dec 15; Vol. 4 (6). Date of Electronic Publication: 2016 Dec 15.