Design principles for cyclin K molecular glue degraders.

Autor: Kozicka Z; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.; Department of Biology, University of Basel, Basel, Switzerland., Suchyta DJ; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.; Department of Chemistry, University of Basel, Basel, Switzerland., Focht V; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland., Kempf G; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland., Petzold G; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.; Monte Rosa Therapeutics, Basel, Switzerland., Jentzsch M; Institute of Clinical Chemistry and Clinical Pharmacology, University and University Hospital Bonn, Bonn, Germany., Zou C; Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Yale University, New Haven, CT, USA., Di Genua C; Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.; VantAI, New York, NY, USA., Donovan KA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA., Coomar S; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland., Cigler M; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria., Mayor-Ruiz C; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.; IRB Barcelona-Institute for Research in Biomedicine, The Barcelona Institute of Science and Technology, Barcelona, Spain., Schmid-Burgk JL; Institute of Clinical Chemistry and Clinical Pharmacology, University and University Hospital Bonn, Bonn, Germany., Häussinger D; Department of Chemistry, University of Basel, Basel, Switzerland., Winter GE; CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria., Fischer ES; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA., Słabicki M; Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA., Gillingham D; Department of Chemistry, University of Basel, Basel, Switzerland., Ebert BL; Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.; Howard Hughes Medical Institute, Boston, MA, USA., Thomä NH; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland. nicolas.thoma@fmi.ch.

Publikováno v: Nature chemical biology [Nat Chem Biol] 2024 Jan; Vol. 20 (1), pp. 93-102. Date of Electronic Publication: 2023 Sep 07.

IKKβ primes inflammasome formation by recruiting NLRP3 to the trans-Golgi network.

Autor: Schmacke NA; Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany., O'Duill F; Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany., Gaidt MM; Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany., Szymanska I; Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany., Kamper JM; Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany., Schmid-Burgk JL; Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany., Mädler SC; Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany., Mackens-Kiani T; Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany., Kozaki T; Singapore Immunology Network (SIgN), Agency for Science, Technology & Research (A∗STAR), 8A Biomedical Grove, Immunos Building #3-4, Biopolis, Singapore 138648, Singapore., Chauhan D; Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany., Nagl D; Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany., Stafford CA; Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany., Harz H; Faculty of Biology, Human Biology and BioImaging, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany., Fröhlich AL; Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany., Pinci F; Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany., Ginhoux F; Singapore Immunology Network (SIgN), Agency for Science, Technology & Research (A∗STAR), 8A Biomedical Grove, Immunos Building #3-4, Biopolis, Singapore 138648, Singapore; Shanghai Institute of Immunology, Shanghai JiaoTong University School of Medicine, 280 South Chongqing Road, Shanghai 200025, China; Translational Immunology Institute, SingHealth Duke-NUS Academic Medical Centre, Singapore 169856, Singapore., Beckmann R; Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany., Mann M; Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany., Leonhardt H; Faculty of Biology, Human Biology and BioImaging, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany., Hornung V; Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany. Electronic address: hornung@genzentrum.lmu.de.

Publikováno v: Immunity [Immunity] 2022 Dec 13; Vol. 55 (12), pp. 2271-2284.e7. Date of Electronic Publication: 2022 Nov 15.

Author Correction: LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding.

Autor: Ludwig KU; Institute of Human Genetics, University of Bonn and University Hospital Bonn, Bonn, Germany., Schmithausen RM; Institute of Hygiene and Public Health, University of Bonn and University Hospital Bonn, Bonn, Germany., Li D; Broad Institute of MIT and Harvard, Massachusetts Institute of Technology, Cambridge, MA, USA.; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Jacobs ML; Institute of Clinical Chemistry and Clinical Pharmacology, University of Bonn and University Hospital Bonn, Bonn, Germany.; Center for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany., Hollstein R; Institute of Human Genetics, University of Bonn and University Hospital Bonn, Bonn, Germany., Blumenstock K; Institute of Clinical Chemistry and Clinical Pharmacology, University of Bonn and University Hospital Bonn, Bonn, Germany., Liebing J; Institute of Experimental Oncology, University of Bonn and University Hospital Bonn, Bonn, Germany., Słabicki M; Broad Institute of MIT and Harvard, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.; Division of Translational Medical Oncology, German Cancer Research Center (DKFZ) and National Center for Tumor Diseases (NCT), Heidelberg, Germany., Ben-Shmuel A; Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel., Israeli O; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel., Weiss S; Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel., Ebert TS; Institute of Clinical Chemistry and Clinical Pharmacology, University of Bonn and University Hospital Bonn, Bonn, Germany., Paran N; Department of Infectious Diseases, Israel Institute for Biological Research, Ness Ziona, Israel., Rüdiger W; Institute of Clinical Chemistry and Clinical Pharmacology, University of Bonn and University Hospital Bonn, Bonn, Germany., Wilbring G; Institute of Hygiene and Public Health, University of Bonn and University Hospital Bonn, Bonn, Germany., Feldman D; Department of Biochemistry and Institute for Protein Design, University of Washington, Seattle, WA, USA., Lippke B; Institute of Human Genetics, University of Bonn and University Hospital Bonn, Bonn, Germany., Ishorst N; Institute of Human Genetics, University of Bonn and University Hospital Bonn, Bonn, Germany.; Institute of Anatomy, Division of Neuroanatomy, University of Bonn and University Hospital Bonn, Bonn, Germany., Hochfeld LM; Institute of Human Genetics, University of Bonn and University Hospital Bonn, Bonn, Germany., Beins EC; Institute of Human Genetics, University of Bonn and University Hospital Bonn, Bonn, Germany., Kaltheuner IH; Institute of Structural Biology, University of Bonn and University Hospital Bonn, Bonn, Germany., Schmitz M; Institute of Structural Biology, University of Bonn and University Hospital Bonn, Bonn, Germany., Wöhler A; Department of General, Visceral and Thoracic Surgery, Bundeswehr Central Hospital Koblenz, Koblenz, Germany., Döhla M; Institute of Hygiene and Public Health, University of Bonn and University Hospital Bonn, Bonn, Germany.; Department of Microbiology and Hospital Hygiene, Bundeswehr Central Hospital Koblenz, Koblenz, Germany., Sib E; Institute of Hygiene and Public Health, University of Bonn and University Hospital Bonn, Bonn, Germany., Jentzsch M; Institute of Clinical Chemistry and Clinical Pharmacology, University of Bonn and University Hospital Bonn, Bonn, Germany., Moench EC; MVZ Laboratory, Dr. Quade and Associates, Cologne, Germany., Borrajo JD; Broad Institute of MIT and Harvard, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Strecker J; Broad Institute of MIT and Harvard, Massachusetts Institute of Technology, Cambridge, MA, USA.; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Reinhardt J; Institute of Experimental Oncology, University of Bonn and University Hospital Bonn, Bonn, Germany., Cleary B; Broad Institute of MIT and Harvard, Massachusetts Institute of Technology, Cambridge, MA, USA., Geyer M; Institute of Structural Biology, University of Bonn and University Hospital Bonn, Bonn, Germany., Hölzel M; Institute of Experimental Oncology, University of Bonn and University Hospital Bonn, Bonn, Germany., Macrae R; Broad Institute of MIT and Harvard, Massachusetts Institute of Technology, Cambridge, MA, USA.; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Nöthen MM; Institute of Human Genetics, University of Bonn and University Hospital Bonn, Bonn, Germany., Hoffmann P; Institute of Human Genetics, University of Bonn and University Hospital Bonn, Bonn, Germany.; Genomics Research Group, Department of Biomedicine, University of Basel, Basel, Switzerland., Exner M; Institute of Hygiene and Public Health, University of Bonn and University Hospital Bonn, Bonn, Germany., Regev A; Broad Institute of MIT and Harvard, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.; Klarman Cell Observatory, Massachusetts Institute of Technology, Cambridge, MA, USA.; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Howard Hughes Medical Institute, Cambridge, MA, USA.; Genentech, South San Francisco, CA, USA., Zhang F; Broad Institute of MIT and Harvard, Massachusetts Institute of Technology, Cambridge, MA, USA.; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Howard Hughes Medical Institute, Cambridge, MA, USA., Schmid-Burgk JL; Broad Institute of MIT and Harvard, Massachusetts Institute of Technology, Cambridge, MA, USA. jonathan.schmid-burgk@ukbonn.de.; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, USA. jonathan.schmid-burgk@ukbonn.de.; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA. jonathan.schmid-burgk@ukbonn.de.; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. jonathan.schmid-burgk@ukbonn.de.; Institute of Clinical Chemistry and Clinical Pharmacology, University of Bonn and University Hospital Bonn, Bonn, Germany. jonathan.schmid-burgk@ukbonn.de.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2022 Aug; Vol. 40 (8), pp. 1295.

Structure-guided multivalent nanobodies block SARS-CoV-2 infection and suppress mutational escape.

Autor: Koenig PA; Core Facility Nanobodies, Medical Faculty, University of Bonn, 53127 Bonn, Germany. pakoenig@uni-bonn.de martin.hallberg@ki.se nicwu@illinois.edu fschmidt@uni-bonn.de.; Institute of Innate Immunity, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Das H; Department of Cell and Molecular Biology, Karolinska Institutet, 17177 Stockholm, Sweden., Liu H; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Kümmerer BM; Institute of Virology, Medical Faculty, University of Bonn, 53127 Bonn, Germany.; German Centre for Infection Research (DZIF), partner site Bonn-Cologne, 53127 Bonn, Germany., Gohr FN; Institute of Innate Immunity, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Jenster LM; Institute of Innate Immunity, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Schiffelers LDJ; Institute of Innate Immunity, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Tesfamariam YM; Institute of Innate Immunity, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Uchima M; Institute of Innate Immunity, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Wuerth JD; Institute of Innate Immunity, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Gatterdam K; Institute of Structural Biology, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Ruetalo N; Institute for Medical Virology and Epidemiology, Section Molecular Virology, University Hospital Tübingen, 72076 Tübingen, Germany., Christensen MH; Institute of Innate Immunity, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Fandrey CI; Institute of Innate Immunity, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Normann S; Institute of Innate Immunity, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Tödtmann JMP; Core Facility Nanobodies, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Pritzl S; Core Facility Nanobodies, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Hanke L; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, 17177 Stockholm, Sweden., Boos J; Institute of Human Genetics, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Yuan M; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Zhu X; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Schmid-Burgk JL; Institute for Clinical Chemistry and Clinical Pharmacology, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Kato H; Institute of Cardiovascular Immunology, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Schindler M; Institute for Medical Virology and Epidemiology, Section Molecular Virology, University Hospital Tübingen, 72076 Tübingen, Germany., Wilson IA; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.; The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Geyer M; Institute of Structural Biology, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Ludwig KU; Institute of Human Genetics, Medical Faculty, University of Bonn, 53127 Bonn, Germany., Hällberg BM; Department of Cell and Molecular Biology, Karolinska Institutet, 17177 Stockholm, Sweden. pakoenig@uni-bonn.de martin.hallberg@ki.se nicwu@illinois.edu fschmidt@uni-bonn.de.; Centre for Structural Systems Biology (CSSB) and Karolinska Institutet VR-RÅC, Notkestrasse 85, 22607 Hamburg, Germany., Wu NC; Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. pakoenig@uni-bonn.de martin.hallberg@ki.se nicwu@illinois.edu fschmidt@uni-bonn.de.; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.; Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA., Schmidt FI; Core Facility Nanobodies, Medical Faculty, University of Bonn, 53127 Bonn, Germany. pakoenig@uni-bonn.de martin.hallberg@ki.se nicwu@illinois.edu fschmidt@uni-bonn.de.; Institute of Innate Immunity, Medical Faculty, University of Bonn, 53127 Bonn, Germany.

Publikováno v: Science (New York, N.Y.) [Science] 2021 Feb 12; Vol. 371 (6530). Date of Electronic Publication: 2021 Jan 12.

Highly Parallel Profiling of Cas9 Variant Specificity.

Autor: Schmid-Burgk JL; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Gao L; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Li D; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Gardner Z; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Strecker J; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Lash B; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Zhang F; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Cambridge, MA 02139, USA. Electronic address: zhang@broadinstitute.org.

Publikováno v: Molecular cell [Mol Cell] 2020 May 21; Vol. 78 (4), pp. 794-800.e8. Date of Electronic Publication: 2020 Mar 17.