Výsledky vyhledávání - "Schineller, J."

Effect of Metal-Ligand Mutations on Phosphoryl Transfer Reactions Catalyzed by Escherichia coli Glutamine Synthetase

Autor: Joseph J. Villafranca, Schineller J, Keck Pj, Lynn M. Abell

Publikováno v: Biochemistry. 34:16695-16702

Glutamine synthetase (GS) converts glutamate to glutamine in the presence of ATP and ammonia and requires two divalent metal ions, designated n1 and n2, for catalysis. The first intermediate, gamma-glutamyl phosphate, is formed during catalysis by th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97753d02990222c895bcbd0efbd65628
https://doi.org/10.1021/bi00051a018

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Regeneration of catalytic activity of glutamine synthetase mutants by chemical activation: exploration of the role of arginines 339 and 359 in activity

Autor: Dhalla, A. M., Li, B., Alibhai, M. F., Yost, K. J., Hemmingsen, J. M., Atkins, W. M., Schineller, J., Villafranca, J. J.

In order to understand the nature of ATP and L-glutamate binding to glutamine synthetase, and the involvement of Arg 339 and Arg 359 in catalysis, these amino acids were changed to cysteine via site-directed mutagenesis. Individual mutations (Arg-->C

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d07384ac3485e3507070b374e0059a14
https://europepmc.org/articles/PMC2142696/