Comparison of two laboratory-developed PCR methods for the diagnosis of Pulmonary Tuberculosis in Brazilian patients with and without HIV infection

Autor: Netto Antonio, Ribeiro Marta, Rupenthal Rubia, Michelon Candice T, Cafrune Patrícia I, Jarczewski Carla, Sperhacke Rosa D, Scherer Luciene C, Rossetti Maria LR, Kritski Afrânio L

Publikováno v: BMC Pulmonary Medicine, Vol 11, Iss 1, p 15 (2011)

Abstract Background Direct smear examination with Ziehl-Neelsen (ZN) staining for the diagnosis of pulmonary tuberculosis (PTB) is cheap and easy to use, but its low sensitivity is a major drawback, particularly in HIV seropositive patients. As such,

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Low prevalence of primary antiretroviral resistance mutations and predominance of HIV-1 clade C at polymerase gene in newly diagnosed individuals from south Brazil

Autor: Rodrigues, Rosangela¹ rohc@rcp-hiv.org, Scherer, Luciene C.², Oliveira, Cristina M.¹, Franco, Heitor Moreira¹, Sperhacke, Rosa Dea³, Paula Ferreira, Joao Leandro¹, Castro, Simone Martins², Stella, Isete Maria⁴, Macedo Brigido, Luis Fernando¹

Publikováno v: Virus Research. Mar2006, Vol. 116 Issue 1/2, p201-207. 7p.

Usefulness of the polymerase chain reaction dot-blot assay, used with Ziehl-Neelsen staining, for the rapid and convenient diagnosis of pulmonary tuberculosis in human immunodeficiency virus-seropositive and -seronegative individuals.

Autor: Scherer LC; Programa de Pós Graduação em Ciências Biológicas - Bioquímica, Universidade Federal do Rio Grande do Sul -UFRGS, Porto Alegre, RS, Brazil; ; Universidade Luterana do Brasil-ULBRA, Canoas, RS, Brazil;, Sperhacke RD; Centro de Desenvolvimento de Ciência e Tecnologia-CDCT, Fundação Estadual de Produção e Pesquisa em Saúde-FEPPS/RS, Porto Alegre, RS, Brazil;, Rossetti ML; Centro de Desenvolvimento de Ciência e Tecnologia-CDCT, Fundação Estadual de Produção e Pesquisa em Saúde-FEPPS/RS, Porto Alegre, RS, Brazil; ; Universidade Luterana do Brasil-ULBRA, Canoas, RS, Brazil;, Ruffino-Netto A; Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil;, Kritski AL; Unidade de Pesquisa em Tuberculose, Programa Acadêmico de TB, Hospital Universitário Clementino Fraga Filho, UPT, HUCFF, Universidade Federal do Rio de Janeiro-UFRJ, Rio de Janeiro, Brazil.

Publikováno v: Infectious disease reports [Infect Dis Rep] 2011 Mar 24; Vol. 3 (1), pp. e3. Date of Electronic Publication: 2011 Mar 24 (Print Publication: 2011).