Compact SchCas9 Recognizes the Simple NNGR PAM

Autor: Shuai Wang, Huilin Mao, Linghui Hou, Ziying Hu, Yao Wang, Tao Qi, Chen Tao, Yuan Yang, Chengdong Zhang, Miaomiao Li, Huihui Liu, Shijun Hu, Renjie Chai, Yongming Wang

Publikováno v: Advanced Science, Vol 9, Iss 4, Pp n/a-n/a (2022)

Abstract Clustered regularly interspaced short palindromic repeat (CRISPR)/SaCas9 is the most popular tool for in vivo genome editing due to its high efficiency and small genome. The authors previously developed four SaCas9 orthologs as genome‐edit

Externí odkaz: https://doaj.org/article/65cf145de86a4625822dec403823eaab

Compact SchCas9 Recognizes the Simple NNGR PAM.

Autor: Wang S; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China., Mao H; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China., Hou L; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China., Hu Z; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China., Wang Y; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China., Qi T; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China., Tao C; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China., Yang Y; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China., Zhang C; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China., Li M; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China., Liu H; Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing, 102300, China., Hu S; Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou, 215000, China., Chai R; State Key Laboratory of Bioelectronics, School of Life Sciences and Technology, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, 210096, China.; Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, China., Wang Y; State Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Fudan University, Shanghai, 200438, China.; Shanghai Engineering Research Center of Industrial Microorganisms, Shanghai, 200438, China.

Publikováno v: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2022 Feb; Vol. 9 (4), pp. e2104789. Date of Electronic Publication: 2021 Dec 06.