Zobrazeno 1 - 10
of 221
pro vyhledávání: '"Sayaka WAKAYAMA"'
Autor:
Mizuki Sakamoto, Aoi Ito, Sayaka Wakayama, Hiroyuki Sasaki, Teruhiko Wakayama, Takashi Ishiuchi
Publikováno v:
Cell Reports, Vol 43, Iss 4, Pp 114118- (2024)
Summary: Zygotic genome activation (ZGA) after fertilization enables the maternal-to-zygotic transition. However, the global view of ZGA, particularly at initiation, is incompletely understood. Here, we develop a method to capture and sequence newly
Externí odkaz:
https://doaj.org/article/9ed8ec9cff2f42ee8f95fda64a19b2b8
Time-lapse observation of mouse preimplantation embryos using a simple closed glass capillary method
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-10 (2023)
Abstract Time-lapse observation is a popular method for analyzing mammalian preimplantation embryos, but it often requires expensive equipment and skilled techniques. We previously developed a simply and costly embryo-culture system in a sealed tube
Externí odkaz:
https://doaj.org/article/c4a958b296f949a7815fb97e7624666a
Publikováno v:
The Journal of Reproduction and Development, Vol 69, Iss 4, Pp 198-205 (2023)
Although freeze-drying sperm can save space, reduce maintenance costs, and facilitate the transportation of genetic samples, the current method requires breakable, custom-made, and expensive glass ampoules. In the present study, we developed a simple
Externí odkaz:
https://doaj.org/article/e66f5b0118a8496ab3d36164ee93d6d2
Autor:
Kohei TORIKAI, Kazuma SHIMIZU, Hiroaki NAGATOMO, Mariko KASAI, Megumi KATO-ITOH, Yuko KAMADA, Ikue SHIBASAKI, Hyojung JEON, Riko KIKUCHI, Sayaka WAKAYAMA, Fabian SUCHY, Hiromitsu NAKAUCHI, Teruhiko WAKAYAMA, Eiji MIZUTANI
Publikováno v:
The Journal of Reproduction and Development, Vol 69, Iss 1, Pp 48-52 (2022)
We examined various methods to enhance the accessibility of intracytoplasmic sperm injection (ICSI) technology to more users by making the technique easier, more efficient, and practical. First, the methods for artificially removing the mouse sperm t
Externí odkaz:
https://doaj.org/article/6b8968a10c4f48a99e0d068d11834145
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-8 (2022)
Abstract Mouse cloning by nuclear transfer using freeze-drying (FD) somatic cells is now possible, but the success rate is significantly lower than that of FD spermatozoa. Because spermatozoa, unlike somatic cells, are haploid cells with hardened nuc
Externí odkaz:
https://doaj.org/article/4cdbbf85b7fe43fcabd41442eae8b7c0
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-9 (2022)
The development of safe preservation methods for genetic resources is important. Here, the authors successfully produce cloned mice from freeze-dried somatic cells, demonstrating the possibility of safe and low-cost preservation of genetic resources.
Externí odkaz:
https://doaj.org/article/a9fbc47c206440e687a4263bcb2a5024
Autor:
Masatoshi Ooga, Rei Inoue, Kousuke Kazama, Sayaka Wakayama, Satoshi Kamimura, Teruhiko Wakayama
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-9 (2022)
Quantification of chromatin dynamics in paternal and maternal pronuclei reveals opposite effects on chromatin dynamics by oocyte- and sperm derived factors.
Externí odkaz:
https://doaj.org/article/ed55195f1ba44d5e8dcedbd8224f2d5a
Autor:
Natsuki USHIGOME, Sayaka WAKAYAMA, Kango YAMAJI, Daiyu ITO, Masatoshi OOGA, Teruhiko WAKAYAMA
Publikováno v:
The Journal of Reproduction and Development, Vol 68, Iss 4, Pp 262-270 (2022)
Freeze-dried sperm (FD sperm) are of great value because they can be stored at room temperature for long periods of time, However, the birth rate of offspring derived from FD sperm is low and the step in the freeze-drying process particularly respons
Externí odkaz:
https://doaj.org/article/1bfb517fa31e4688ac0109d6313d0cbb
Autor:
Erika HAYASHI, Sayaka WAKAYAMA, Daiyu ITO, Ayumi HASEGAWA, Keiji MOCHIDA, Masatoshi OOGA, Atsuo OGURA, Teruhiko WAKAYAMA
Publikováno v:
The Journal of Reproduction and Development, Vol 68, Iss 2, Pp 118-124 (2022)
Mammalian embryos are most commonly cryopreserved in liquid nitrogen; however, liquid nitrogen is not available in special environments, such as the International Space Station (ISS), and vitrified embryos must be stored at −80°C. Recently, the hi
Externí odkaz:
https://doaj.org/article/f87a18adb9764d4a96fb8d8d79088e4e
Publikováno v:
The Journal of Reproduction and Development, Vol 66, Iss 5, Pp 427-433 (2020)
Mouse oocytes are generally collected after euthanasia. However, if oocytes were collected without euthanasia, then mice could be used to collect oocytes again after recovery. This condition is especially useful for mice that are genotypically rare.
Externí odkaz:
https://doaj.org/article/dedc9b39da8447b089e4644615877c24