Computational engineering of previously crystallized pyruvate formate-lyase activating enzyme reveals insights into SAM binding and reductive cleavage.

Autor: Moody JD; Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah, USA; Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, USA., Hill S; Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, USA., Lundahl MN; Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, USA., Saxton AJ; Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah, USA., Galambas A; Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, USA., Broderick WE; Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, USA., Lawrence CM; Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, USA., Broderick JB; Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, USA. Electronic address: jbroderick@montana.edu.

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2023 Jun; Vol. 299 (6), pp. 104791. Date of Electronic Publication: 2023 May 06.