Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Saurabh Nirantar"'
Autor:
Trina Faye Osothprarop, Maybelle K. Go, Wen Shan Yew, Saurabh Nirantar, Alexander G Karabadzhak, Sergio G. Peisajovich, Jasmine Puay Suan Chua, Mcdonald Seth
Publikováno v:
ACS Synthetic Biology. 9:1725-1735
Terminal deoxynucleotidyl transferase (TdT) catalyzes template free incorporation of arbitrary nucleotides onto single-stranded DNA. Due to this unique feature, TdT is widely used in biotechnology and clinical applications. One particularly tantalizi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c103693f08799c4a1c60f0cd8e4c3fcd
https://doi.org/10.1021/acssynbio.0c00078
https://doi.org/10.1021/acssynbio.0c00078
Autor:
Saurabh Nirantar
Publikováno v:
Molecules
Molecules, Vol 26, Iss 5599, p 5599 (2021)
Molecules, Vol 26, Iss 5599, p 5599 (2021)
Enzymes underpin the processes required for most biotransformations. However, natural enzymes are often not optimal for biotechnological uses and must be engineered for improved activity, specificity and stability. A rich and growing variety of wet-l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e15547bc48cf7568b75b72efe423f456
https://pubmed.ncbi.nlm.nih.gov/34577070
https://pubmed.ncbi.nlm.nih.gov/34577070
Publikováno v:
Trends in biotechnology. 39(10)
The field of storing information in DNA has expanded exponentially. Most common modalities involve encoding information from bits into synthesized nucleotides, storage in liquid or dry media, and decoding via sequencing. However, limitations to this
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c727075fea0eca12c75a90435e9b5c87
https://pubmed.ncbi.nlm.nih.gov/33455842
https://pubmed.ncbi.nlm.nih.gov/33455842
Publikováno v:
Protein Expression and Purification. 129:18-24
Protein purification typically involves expressing a recombinant gene comprising a target protein fused to a suitable affinity tag. After purification, it is often desirable to remove the affinity tag to prevent interference with downstream functions
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3edfd684875a801225394cce02a25c07
https://doi.org/10.1016/j.pep.2016.09.001
https://doi.org/10.1016/j.pep.2016.09.001
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1596
Peptide motifs are crucial mediators of protein-protein interactions as well as sites of specific protease activity. The detection and characterization of these events is therefore indispensable for a detailed understanding of cellular regulation. He
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::3ce7bd5b5d9faab666b0bf8ddcd6cd94
https://pubmed.ncbi.nlm.nih.gov/28293887
https://pubmed.ncbi.nlm.nih.gov/28293887
Publikováno v:
Methods in Molecular Biology ISBN: 9781493969388
Peptide motifs are crucial mediators of protein-protein interactions as well as sites of specific protease activity. The detection and characterization of these events is therefore indispensable for a detailed understanding of cellular regulation. He
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f8b572989d1ebc9e99d9c0e26430b618
https://doi.org/10.1007/978-1-4939-6940-1_11
https://doi.org/10.1007/978-1-4939-6940-1_11
Publikováno v:
Cell Cycle. 7:2960-2966
Centrosomes (spindle pole body in yeast) constitute the two poles of the bipolar mitotic spindle and play a prominent role in the segregation of chromosomes during mitosis. Like chromosomes, the centrosome inherited from the progenitor cell duplicate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3781b212fa75c7dd9b655c3ef055c7f
https://doi.org/10.4161/cc.7.19.6783
https://doi.org/10.4161/cc.7.19.6783
DNA Replication Checkpoint Prevents Precocious Chromosome Segregation by Regulating Spindle Behavior
Publikováno v:
Molecular Cell. 16(5):687-700
The DNA replication checkpoint maintains replication fork integrity and prevents chromosome segregation during replication stresses. Mec1 and Rad53 (human ATM/ATR- and Chk2-like kinases, respectively) are critical effectors of this pathway in yeast.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cfab2916449ffc437bd4266153448d2
Publikováno v:
Biosensorsbioelectronics. 56
We have previously developed a sensitive and modular homogenous biosensor system using peptides to detect target ligands. By transposing the basic mechanistic principle of the nuclease protection assay into this biosensor framework, we have developed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ded48095d05f84170c3cb2ba67830be1
https://pubmed.ncbi.nlm.nih.gov/24508816
https://pubmed.ncbi.nlm.nih.gov/24508816
Publikováno v:
Biosensorsbioelectronics. 47
Numerous peptide ligands including protease recognition sequences, peptides mediating protein–protein interactions, peptide epitopes of antibodies and mimotopes are available which bind molecules of interest. However, there is currently no facile m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3af853697e682b45b9ddda09c13faa7b
https://pubmed.ncbi.nlm.nih.gov/23612064
https://pubmed.ncbi.nlm.nih.gov/23612064