Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Saumen Poddar"'
Publikováno v:
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1211
Ultrafast affinity extraction (UAE) and affinity microcolumns containing immobilized human serum albumin (HSA) were employed to evaluate the effect of advanced stage glycation on HSA and its binding to warfarin, a common site-specific probe for Sudlo
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2466
Entrapment is a noncovalent immobilization method that enables a large biological binding agent, such as a protein, to be put within a support without modifying the structure of the binding agent. This chapter describes an on-column entrapment method
Publikováno v:
Methods in Molecular Biology ISBN: 9781071621752
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d215cb7f6f217cb5854313a7730302c7
https://doi.org/10.1007/978-1-0716-2176-9_14
https://doi.org/10.1007/978-1-0716-2176-9_14
Publikováno v:
Analytica Chimica Acta. 1239:340629
Publikováno v:
Electrophoresis
Affinity monolith chromatography (AMC) is a liquid chromatographic technique that utilizes a monolithic support with a biological ligand or related binding agent to isolate, enrich, or detect a target analyte in a complex matrix. The target-specific
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f9c610b143096a60e4e74fee8623acd
https://europepmc.org/articles/PMC9536602/
https://europepmc.org/articles/PMC9536602/
Autor:
Sazia Iftekhar, Morgan Walters, Ashley G. Woolfork, Shae Lott, Susan T. Ovbude, David S. Hage, Elliott Rodriguez, Allegra Pekarek, Saumen Poddar, Kyungah Suh
Publikováno v:
J Chromatogr B Analyt Technol Biomed Life Sci
The field of affinity chromatography, which employs a biologically-related agent as the stationary phase, has seen significant growth since the modern era of this method began in 1968. This review examines the major developments and trends that have
Publikováno v:
J Chromatogr B Analyt Technol Biomed Life Sci
Several approaches were compared for the entrapment of proteins within hydrazide-activated silica for use in affinity microcolumns and high performance affinity chromatography. Human serum albumin (HSA) and concanavalin A (Con A) were used as model p
Publikováno v:
Handbook of Smart Materials in Analytical Chemistry
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1815dbee1b906406465fb6c13753125b
https://doi.org/10.1002/9781119422587.ch7
https://doi.org/10.1002/9781119422587.ch7
Publikováno v:
J Chromatogr B Analyt Technol Biomed Life Sci
An on-line purification and entrapment system was developed that could extract a protein from a sample such as serum and entrap this protein within a small column for use in high-performance affinity chromatography. Human serum albumin (HSA) was empl
Publikováno v:
Methods (San Diego, Calif.). 146
Many biological processes involve solute-protein interactions and solute-solute competition for protein binding. One method that has been developed to examine these interactions is zonal elution affinity chromatography. This review discusses the theo