Zobrazeno 1 - 10
of 174
pro vyhledávání: '"Satoshi Inouye"'
Autor:
Satoshi Inouye, Jun-ichi Sato, Yuiko Sahara-Miura, Yuri Tomabechi, Yuto Sumida, Shun-ichi Sekine, Mikako Shirouzu, Takamitsu Hosoya
Publikováno v:
PLoS ONE, Vol 17, Iss 9 (2022)
Native Oplophorus luciferase (OpLase) and its catalytic 19 kDa protein (wild KAZ) show highest luminescence activity with coelenterazine (CTZ) among CTZ analogs. Mutated wild KAZ with 16 amino acid substitutions (nanoKAZ/nanoLuc) utilizes bis-coelent
Externí odkaz:
https://doaj.org/article/0ab9804ceac14a5fa5afb2bd5e36d483
Autor:
Satoshi Inouye, Yuto Sumida, Yuri Tomabechi, Jumpei Taguchi, Mikako Shirouzu, Takamitsu Hosoya
Publikováno v:
PLoS ONE, Vol 16, Iss 6, p e0251743 (2021)
The Ca2+-binding photoprotein aequorin is a complex of apoAequorin (apoprotein) and (S)-2-peroxycoelenterazine. Aequorin can be regenerated by the incubation of apoAequorin with coelenterazine and molecular oxygen (O2). In this study, to investigate
Externí odkaz:
https://doaj.org/article/3249aef8b5a3499cbe153a480cac8bb5
Autor:
Satoru Yokawa, Takahiro Suzuki, Ayumi Hayashi, Satoshi Inouye, Yoshikazu Inoh, Tadahide Furuno
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 6 (2018)
Degranulation refers to the secretion of inflammatory mediators, such as histamine, serotonin, and proteases, that are stored within the granules of mast cells and that trigger allergic reactions. The amount of these released mediators has been measu
Externí odkaz:
https://doaj.org/article/743f9d758829421d832bc32565dbc960
Publikováno v:
PLoS ONE, Vol 8, Iss 12, p e84023 (2013)
Bioluminescence in fireflies and click beetles is produced by a luciferase-luciferin reaction. The luminescence property and protein structure of firefly luciferase have been investigated, and its cDNA has been used for various assay systems. The che
Externí odkaz:
https://doaj.org/article/588c3ff329e94f15be39e163423f8ced
Publikováno v:
PLoS ONE, Vol 6, Iss 9, p e25243 (2011)
BACKGROUND: Matrix metalloproteinase-2 (MMP-2) plays an important role in cancer progression and metastasis. MMP-2 is secreted as a pro-enzyme, which is activated by the membrane-bound proteins, and the polarized distribution of secretory and the mem
Externí odkaz:
https://doaj.org/article/83496556571e44e5b1362fdac63a2dc5
Publikováno v:
Biochemical and Biophysical Research Communications. 665:133-140
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5f02f4b0c43ee1ab2a3deaf5db76249c
https://doi.org/10.1016/j.bbrc.2023.05.007
https://doi.org/10.1016/j.bbrc.2023.05.007
Publikováno v:
Photochemistry and Photobiology. 98:1068-1076
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::78144b90a8bb18fc12cbf97d812a1dbe
https://doi.org/10.1111/php.13590
https://doi.org/10.1111/php.13590
Autor:
Honami Sugimoto, Shinji Fukuda, Satoru Yokawa, Miki Hori, Hotsuna Ninomiya, Takuma Sato, Ken Miyazawa, Tatsushi Kawai, Tadahide Furuno, Satoshi Inouye, Shigemi Goto, Takahiro Suzuki
Publikováno v:
Biochemical and biophysical research communications. 635
The secretions of osteocalcin and bone morphogenetic protein 2 (BMP2) from living osteoblastic cells were visualized for the first time using a method of video-rate bioluminescence imaging. The fusion proteins with Gaussia luciferase (GLase) for mous
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4db02cfc80329ea701f8fca51cb79ef9
https://pubmed.ncbi.nlm.nih.gov/36279682
https://pubmed.ncbi.nlm.nih.gov/36279682
Publikováno v:
Biochemical and Biophysical Research Communications. 529:678-684
In Cypridina (Vargula) hilgendorfii, Cypridina luciferin is converted from Cypridina luciferyl sulfate by a sulfotransferase with adenosine 3′, 5′-diphosphate (PAP), and is used for the luminescence reaction of Cypridina luciferase. We found that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f4c0dc85a5e7cf26acbf99d7901ab9f
https://doi.org/10.1016/j.bbrc.2020.05.167
https://doi.org/10.1016/j.bbrc.2020.05.167
Publikováno v:
YAKUGAKU ZASSHI. 140:969-977
We developed a method of video-rate bioluminescence imaging to visualize proteins secreted from living cells. A protein of interest was fused to Gaussia luciferase (GLase), and the luminescence signals of secreted GLase with coelenterazine (luciferin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5c44627b420d75c03c02d51c7c34d3b
https://doi.org/10.1248/yakushi.20-00012-2
https://doi.org/10.1248/yakushi.20-00012-2