Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Satoru Sekiya"'
Autor:
Takashi Yokoyama, Fumiko Nishikawa, Satoru Sekiya, Satoshi Nishikawa, Penmetcha K. R. Kumar, Ken Noda
Publikováno v:
The Journal of Biochemistry. 139:383-390
In order to isolate RNA aptamers against the mouse prion protein (mPrP), we carried out in vitro selection from RNA pools containing a 30-nucleotide randomized region. Aptamer 60-3 was found to have a high affinity for mPrP (K(d) = 5.6 +/- 1.5 nM), a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da9fcf94c836a55ed9c1a1ea193a225a
https://doi.org/10.1093/jb/mvj046
https://doi.org/10.1093/jb/mvj046
Autor:
Satoshi Nishikawa, Kazunari Taira, Kunitada Shimotohno, Satoru Sekiya, Penmetcha K. R. Kumar, Nobuko Kakiuchi, Kotaro Fukuda, Joonsung Hwang, Daesety Vishnuvardhan
Publikováno v:
European Journal of Biochemistry. 267:3685-3694
Nonstructural protein 3 (NS3) from hepatitis C virus (HCV) is a serine protease that provides an essential function in maturation of the virus by cleaving the nonstructural regions of the viral polyprotein. The goal of this work was to isolate RNA ap
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::96543116659408f33902f1a1ad34cc41
https://doi.org/10.1046/j.1432-1327.2000.01400.x
https://doi.org/10.1046/j.1432-1327.2000.01400.x
Autor:
Yuzuru Ikehara, Yasuhito Tanaka, Atsushi Kuno, Hisashi Narimatsu, Satoru Sekiya, Kiyoaki Ito, Atsushi Matsuda, Masashi Mizokami, Michiie Sakamoto, Shuhei Hige, Masayoshi Kage
Publikováno v:
Scientific Reports
Although liver fibrosis reflects disease severity in chronic hepatitis patients, there has been no simple and accurate system to evaluate the therapeutic effect based on fibrosis. We developed a glycan-based immunoassay, FastLec-Hepa, to fill this un
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3d882f51c222dfea355fa71881f9f54
https://pubmed.ncbi.nlm.nih.gov/23323209
https://pubmed.ncbi.nlm.nih.gov/23323209
Autor:
Satoshi Nishikawa, Tsunemi Hasegawa, Satoru Sekiya, Michinori Kohara, Fumiko Nishikawa, Kotaro Fukuda, Takuya Umehara
Publikováno v:
Nucleic acids symposium series (2004). (48)
Nonstructural protein 3 (NS3) of hepatitis C virus (HCV) is a multi-functional enzyme having protease and helicase activities. NS3 is essential for HCV replication and proliferation. Previously, we obtained RNA aptamers against NS3 protease domain (P
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a65228870ebf5bd95d18c4234219cebc
https://pubmed.ncbi.nlm.nih.gov/17150545
https://pubmed.ncbi.nlm.nih.gov/17150545
Publikováno v:
Scopus-Elsevier
Prion disease is caused by conformational change of normal cellular type of prion protein (PrP(c)) folding into abnormal type (PrP(sc)). We succeeded to isolate anti-PrP(c) aptamers. In the presence of competitor RNA, anti-PrP(c) aptamers showed high
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49ddd82c52548a622aef9b05290ddb86
https://pubmed.ncbi.nlm.nih.gov/17150783
https://pubmed.ncbi.nlm.nih.gov/17150783
Publikováno v:
Prions ISBN: 4431255397
Since the in vitro selection method, which chooses nucleic acid molecules of demands from huge scale clot of randomized nucleic acid molecules, has been developed (Tuerk, et al., Science.; 249 (4968):505-10, 1990 etc.), various species of functional
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e0017706f024af1ffb3cd57e75d8b47d
https://doi.org/10.1007/4-431-29402-3_26
https://doi.org/10.1007/4-431-29402-3_26
Autor:
Kikuchi Kunio, Kotaro Fukuda, Takuya Umehara, Satoshi Nishikawa, Satoru Sekiya, Tsunemi Hasegawa
Publikováno v:
Biochemical and biophysical research communications. 325(3)
The hepatitis C virus non-structural protein 3 (HCV NS3) possesses both protease and helicase activities that are essential for viral replication. In a previous study, we obtained RNA aptamers that specifically and efficiently inhibited NS3 protease
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95fbb8bd6faa3045558dfb29b7f6cdcb
https://pubmed.ncbi.nlm.nih.gov/15541341
https://pubmed.ncbi.nlm.nih.gov/15541341
Autor:
Isao Kusakabe, Kazunari Taira, Satoru Sekiya, Kotaro Fukuda, Satoshi Nishikawa, Nobuko Kakiuchi, Joonsung Hwang
Publikováno v:
Nucleic acids symposium series. (44)
Non-structural protein 3 (NS3) derived from Hepatitis C virus (HCV) is essential for viral proliferation and has two functional domains; trypsin-like serine protease and helicase. Recently we obtained three types of RNA aptamers (G9-I, -II and -III)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2117853b000113767285e747fb9772c7
https://pubmed.ncbi.nlm.nih.gov/12903319
https://pubmed.ncbi.nlm.nih.gov/12903319
Publikováno v:
Journal of biochemistry. 133(3)
RNA aptamers that bind specifically to hepatitis C virus (HCV) NS3 protease domain (DeltaNS3) were identified in previous studies. These aptamers, G9-I, -II, and -III, were isolated using an in vitro selection method and they share a common loop with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50279097d71c9eafa4ebad3b241c9e88
https://pubmed.ncbi.nlm.nih.gov/12761171
https://pubmed.ncbi.nlm.nih.gov/12761171
Autor:
Satoshi Nishikawa, Nobuko Kakiuchi, Kohei Funaji, Satoru Sekiya, Kotaro Fukuda, Fumiko Nishikawa
Publikováno v:
Nucleic acids research. 31(7)
Non-structural protein 3 (NS3) of hepatitis C virus (HCV) has two distinct activities, protease and helicase, which are essential for HCV proliferation. In previous work, we obtained RNA aptamers (G9-I, II and III) which specifically bound the NS3 pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9baf885eba1bd10d51907d5aaffc7fac
https://pubmed.ncbi.nlm.nih.gov/12655010
https://pubmed.ncbi.nlm.nih.gov/12655010