Protocol to study CCT-mediated folding of Gβ5 by single-particle cryo-EM

Autor: Sass, Mikaila I., Wang, Shuxin, Mack, Deirdre, Cottam, Samuel L., Shen, Peter S., Willardson, Barry M.

Publikováno v: In STAR Protocols 21 June 2024 5(2)

Visualizing the chaperone-mediated folding trajectory of the G protein β5 β-propeller.

Autor: Wang S; Department of Biochemistry, 15 N. Medical Drive East, University of Utah, Salt Lake City, UT, 84112, USA., Sass MI; Department of Chemistry and Biochemistry, C100 BNSN, Brigham Young University, Provo, UT, 84602, USA., Kwon Y; Department of Chemistry and Biochemistry, C100 BNSN, Brigham Young University, Provo, UT, 84602, USA., Ludlam WG; Department of Chemistry and Biochemistry, C100 BNSN, Brigham Young University, Provo, UT, 84602, USA., Smith TM; Department of Chemistry and Biochemistry, C100 BNSN, Brigham Young University, Provo, UT, 84602, USA., Carter EJ; Department of Chemistry and Biochemistry, C100 BNSN, Brigham Young University, Provo, UT, 84602, USA., Gladden NE; Department of Chemistry and Biochemistry, C100 BNSN, Brigham Young University, Provo, UT, 84602, USA., Riggi M; Department of Biochemistry, 15 N. Medical Drive East, University of Utah, Salt Lake City, UT, 84112, USA., Iwasa JH; Department of Biochemistry, 15 N. Medical Drive East, University of Utah, Salt Lake City, UT, 84112, USA., Willardson BM; Department of Chemistry and Biochemistry, C100 BNSN, Brigham Young University, Provo, UT, 84602, USA., Shen PS; Department of Biochemistry, 15 N. Medical Drive East, University of Utah, Salt Lake City, UT, 84112, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2023 May 04. Date of Electronic Publication: 2023 May 04.