Zobrazeno 1 - 10
of 85
pro vyhledávání: '"Sasha F. Levy"'
Autor:
Joseph J. Hale, Takeshi Matsui, Ilan Goldstein, Martin N. Mullis, Kevin R. Roy, Christopher Ne Ville, Darach Miller, Charley Wang, Trevor Reynolds, Lars M. Steinmetz, Sasha F. Levy, Ian M. Ehrenreich
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-15 (2024)
Abstract Interactions between genetic perturbations and segregating loci can cause perturbations to show different phenotypic effects across genetically distinct individuals. To study these interactions on a genome scale in many individuals, we used
Externí odkaz:
https://doaj.org/article/ee32dc78706e4b53b16fa9b04f944fc0
Autor:
Takeshi Matsui, Martin N. Mullis, Kevin R. Roy, Joseph J. Hale, Rachel Schell, Sasha F. Levy, Ian M. Ehrenreich
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Heritable traits can be affected by additive, dominance, and epistatic effects at genetic loci. Here, the authors use chromosomally-encoded barcodes to perform linkage mapping in diploid cross progeny in budding yeast, finding that epistasis in diplo
Externí odkaz:
https://doaj.org/article/ca6975fed8d444fda721b8c44e6948c6
Autor:
Drew S Tack, Peter D Tonner, Abe Pressman, Nathan D Olson, Sasha F Levy, Eugenia F Romantseva, Nina Alperovich, Olga Vasilyeva, David Ross
Publikováno v:
PLoS ONE, Vol 18, Iss 3, p e0283548 (2023)
As synthetic biology expands and accelerates into real-world applications, methods for quantitatively and precisely engineering biological function become increasingly relevant. This is particularly true for applications that require programmed sensi
Externí odkaz:
https://doaj.org/article/021b2a7e505f418e9827dacfaf53e0d0
Autor:
Drew S Tack, Peter D Tonner, Abe Pressman, Nathan D Olson, Sasha F Levy, Eugenia F Romantseva, Nina Alperovich, Olga Vasilyeva, David Ross
Publikováno v:
Molecular Systems Biology, Vol 17, Iss 3, Pp 1-26 (2021)
Abstract Allostery is a fundamental biophysical mechanism that underlies cellular sensing, signaling, and metabolism. Yet a quantitative understanding of allosteric genotype‐phenotype relationships remains elusive. Here, we report the large‐scale
Externí odkaz:
https://doaj.org/article/d9a3618aa3a44816b3647fe677f82e63
Publikováno v:
eLife, Vol 9 (2020)
To characterize how protein-protein interaction (PPI) networks change, we quantified the relative PPI abundance of 1.6 million protein pairs in the yeast Saccharomyces cerevisiae across nine growth conditions, with replication, for a total of 44 mill
Externí odkaz:
https://doaj.org/article/517d256a066046a8be08fb95865a75c2
Autor:
Drew S Tack, Peter D Tonner, Abe Pressman, Nathan D Olson, Sasha F Levy, Eugenia F Romantseva, Nina Alperovich, Olga Vasilyeva, David Ross
Publikováno v:
Molecular Systems Biology, Vol 17, Iss 12, Pp 1-1 (2021)
Externí odkaz:
https://doaj.org/article/d5fb0b7e4c8c419f9342c6766b2f15df
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-9 (2017)
Protein-protein interactions (PPIs) play a major role in defining biological functions. Here, the authors present PPiSeq, a method to quantitatively score dynamic PPIs in yeast combining fragment complementation assays, genomic double-barcoding, and
Externí odkaz:
https://doaj.org/article/a2d1f7e3c1634cb29a0d1f9355ce7b11
Autor:
Justin D Smith, Ulrich Schlecht, Weihong Xu, Sundari Suresh, Joe Horecka, Michael J Proctor, Raeka S Aiyar, Richard A O Bennett, Angela Chu, Yong Fuga Li, Kevin Roy, Ronald W Davis, Lars M Steinmetz, Richard W Hyman, Sasha F Levy, Robert P St.Onge
Publikováno v:
Molecular Systems Biology, Vol 13, Iss 2, Pp 1-15 (2017)
Abstract The low costs of array‐synthesized oligonucleotide libraries are empowering rapid advances in quantitative and synthetic biology. However, high synthesis error rates, uneven representation, and lack of access to individual oligonucleotides
Externí odkaz:
https://doaj.org/article/7ab84b1308cd4c04b4426d5c0715d2a2
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 7, Iss 1, Pp 143-153 (2017)
Systematic screens for genetic interactions are a cornerstone of both network and systems biology. However, most screens have been limited to characterizing interaction networks in a single environment. Moving beyond this static view of the cell requ
Externí odkaz:
https://doaj.org/article/4ff92dad4c8c4aa98976c6b2e4311313
Autor:
Stephanie Lauer, Grace Avecilla, Pieter Spealman, Gunjan Sethia, Nathan Brandt, Sasha F Levy, David Gresham
Publikováno v:
PLoS Biology, Vol 16, Iss 12, p e3000069 (2018)
Copy number variants (CNVs) are a pervasive source of genetic variation and evolutionary potential, but the dynamics and diversity of CNVs within evolving populations remain unclear. Long-term evolution experiments in chemostats provide an ideal syst
Externí odkaz:
https://doaj.org/article/8979b08154c34000bcd0157db9dddafe