Optimized DPPH assay in a detergent-based buffer system for measuring antioxidant activity of proteins

Autor: Sascha C.T. Nicklisch, J. Herbert Waite

Publikováno v: MethodsX, Vol 1, Iss C, Pp 233-238 (2014)

The free radical method using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) is a well established assay for the in vitro determination of antioxidant activity in food and biological extracts. The standard DPPH assay uses methanol or ethanol as solvents, or b

Externí odkaz: https://doaj.org/article/1bbc8d6c7e1344119416ba5f4ebbc4f7

2,4,6-Tribromophenol Exposure Decreases P-Glycoprotein Transport at the Blood-Brain Barrier

Autor: Sascha C.T. Nicklisch, Andrew W Trexler, Ronald E. Cannon, Linda S. Birnbaum, Gabriel A. Knudsen

Publikováno v: Toxicol Sci

2,4,6-Tribromophenol (TBP, CAS No. 118-79-6) is a brominated chemical used in the production of flame-retardant epoxy resins and as a wood preservative. In marine environments, TBP is incorporated into shellfish and consumed by predatory fish. Food p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00229803edadcd3275bf123556a5209c
https://doi.org/10.1093/toxsci/kfz155

Transporter-interfering chemicals inhibit P-glycoprotein of yellowfin tuna (Thunnus albacares)

Autor: Steven D. Rees, Sascha C.T. Nicklisch, Geoffrey Chang, Aaron P. McGrath, Amara Pouv, Amro Hamdoun

Publikováno v: Comparative biochemistry and physiology. Toxicologypharmacology : CBP. 248

Marine pollutants bioaccumulate at high trophic levels of marine food webs and are transferred to humans through consumption of apex species. Yellowfin tuna (Thunnus albacares) are marine predators, and one of largest commercial fisheries in the worl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1af7e200e92933d363a095004e28eb7
https://pubmed.ncbi.nlm.nih.gov/34116183

Redox Capacity of an Extracellular Matrix Protein Associated with Adhesion in Mytilus californianus

Autor: C. Gruian, Sascha C.T. Nicklisch, J. Herbert Waite, Jamie E. Spahn, Hongjun Zhou

Publikováno v: Nicklisch, SCT; Spahn, JE; Zhou, H; Gruian, CM; & Waite, JH. (2016). Redox Capacity of an Extracellular Matrix Protein Associated with Adhesion in Mytilus californianus. Biochemistry, 55(13), 2022-2030. doi: 10.1021/acs.biochem.6b00044. UC Santa Barbara: Retrieved from: http://www.escholarship.org/uc/item/00c6n0ff
Biochemistry, vol 55, iss 13

© 2016 American Chemical Society. Adhesive mussel foot proteins (Mfps) rely in part on DOPA (3,4-dihydroxyphenyl-l-alanine) side chains to mediate attachment to mineral surfaces underwater. Oxidation of DOPA to Dopaquinone (Q) effectively abolishes

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa0eab672838a288ac54e1c8e98ff4dc
https://doi.org/10.1021/acs.biochem.6b00044

Transport in technicolor: Mapping ATP-binding cassette transporters in sea urchin embryos

Autor: Sascha C.T. Nicklisch, Amro Hamdoun, Joseph P. Campanale, Tufan Gökirmak, Lauren E. Shipp

Publikováno v: Molecular Reproduction and Development. 81:778-793

One quarter of eukaryotic genes encode membrane proteins. These include nearly 1,000 transporters that translocate nutrients, signaling molecules, and xenobiotics across membranes. While it is well appreciated that membrane transport is critical for

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5852e31657040bb621764f98fb816861
https://doi.org/10.1002/mrd.22357