Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Sarvamangala V. Prasad"'
Autor:
Sarvamangala V. Prasad, Cecilia Cariño, Eric D. Schwoebel, Sheri M. Skinner, Bonnie S. Dunbar, Mayel Chirinos, Bohn Dunbar, Fernando Larrea
Publikováno v:
Scopus-Elsevier
The mammalian zona pellucida (ZP) consists of three glycoproteins (ZP1, ZP2 and ZP3), which are variably conserved among species at the genomic and amino acid levels. In order to evaluate the expression of ZP during ovarian development, a population
Publikováno v:
Biology of Reproduction. 65:951-960
A lactosaminoglycan-associated antigen is associated with a carbohydrate moiety of all three zona pellucida (ZP) glycoproteins of pig and rabbit but is absent in the mouse and rat. A monoclonal antibody (PS1) recognizing this determinant was obtained
Publikováno v:
Journal of the Society for Gynecologic Investigation. 8:S43-S48
The ovary does not have a distinct morphologic barrier between the immune system and the developing gametes. This is in contrast to the testis in which the junctional complexes between the Sertoli cells form the blood-testis barrier. Whereas there ar
Publikováno v:
Seminars in Reproductive Medicine. 18:141-150
The incidence of infertility related to both male and female factors continues to rise despite many advances in reproductive technologies. Some abnormalities in human gamete interaction have been shown to be due to defects in the sperm, and others ha
Autor:
Cecilia Cariño, N. Wang, Bonnie S. Dunbar, Sarvamangala V. Prasad, J. Cartwright, Sheri M. Skinner
Publikováno v:
Cells Tissues Organs. 166:148-164
The zona pellucida (ZP) is the extracellular matrix that plays important roles in sperm-egg interaction. The ZP is composed of three major glycoproteins that exhibit heterogeneity due to extensive post-translational modifications including glycosylat
Publikováno v:
Biology of Reproduction. 61:1373-1380
Zona pellucida (ZP) glycoproteins contain numerous antigenic determinants including carbohydrate, protein, and conformational epitopes; and the immunogenicity of these complex glycoproteins varies in different mammalian hosts. Studies have now shown
Publikováno v:
Molecular Reproduction and Development. 43:519-529
A cDNA encoding the rabbit 55 kDa ZP protein was expressed using a baculovirus expression system and was evaluated for its ability to elicit antibodies which may interfere with sperm-ZP interaction. The expressed glycosylated protein, BV55, was purif
Publikováno v:
American Journal of Reproductive Immunology. 35:163-174
Publikováno v:
Biology of Reproduction. 52:1167-1178
In the present study we have used a molecular approach to evaluate the immunogenicity and antigenicity of glycosylated and non-glycosylated recombinant rabbit 55-kDa zona pellucida (ZP) protein. The 55-kDa cDNA was expressed in insect (Sf9) cells thr
Autor:
Sarvamangala V. Prasad, Eric D. Schwoebel, P Cheung, Bonnie S. Dunbar, Therese M. Timmons, Richard G. Cook, V H Lee
Publikováno v:
Journal of Biological Chemistry. 268:12412-12417
A cDNA (rc75) encoding a 75-kDa rabbit zona pellucida (ZP) glycoprotein (R75) has been cloned and sequenced. The predicted amino acid sequence consists of 676 amino acids including seven potential N-glycosylation sites. The cDNA hybridizes to a 2.4-k