Výsledky vyhledávání - "Sarita Dhounchak"

Akademický článek

Heparan sulfate proteoglycans in beta cells provide a critical link between endoplasmic reticulum stress, oxidative stress and type 2 diabetes.

Autor: Sarita Dhounchak, Sarah K Popp, Debra J Brown, D Ross Laybutt, Trevor J Biden, Stefan R Bornstein, Christopher R Parish, Charmaine J Simeonovic

Publikováno v: PLoS ONE, Vol 16, Iss 6, p e0252607 (2021)

Heparan sulfate proteoglycans (HSPGs) consist of a core protein with side chains of the glycosaminoglycan heparan sulfate (HS). We have previously identified (i) the HSPGs syndecan-1 (SDC1), and collagen type XVIII (COL18) inside mouse and human isle

Externí odkaz: https://doaj.org/article/68eb3c59062444408e639f2ff92b388d

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Heparan sulfate proteoglycans in beta cells provide a critical link between endoplasmic reticulum stress, oxidative stress and type 2 diabetes

Autor: Sarah K. Popp, Christopher R. Parish, Charmaine J. Simeonovic, Trevor J. Biden, Sarita Dhounchak, D. Ross Laybutt, Debra J. Brown, Stefan R. Bornstein

Publikováno v: PLoS ONE
PLoS ONE, Vol 16, Iss 6, p e0252607 (2021)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93f39bdc0d38a6741497adf8084e0053
https://pubmed.ncbi.nlm.nih.gov/34086738

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Flow cytometry analysis of mouse islet cell expressionof heparan sulfate (HS), heparan sulfate proteoglycans (HSPGs) and heparanase (HPSE) v1

Autor: Sarah Popp, Sarita Dhounchak, Charmaine Simeonovic

Isolated mouse islets were dispersed into single cells using Accutase (Millipore; 250 µl/500 islets. 40,000 islet cells were transferred to individual wells of a 96 well culture plate (CELLSTAR) for staining and flow cytometry analysis or for cultur

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9cb6556970fc372bcf6038b0cddc5e6f
https://doi.org/10.17504/protocols.io.bmsyk6fw

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Isolation of mouse islet cells, culture with heparan sulfate mimetics and flow cytometry analysis of beta cell viability v1

Autor: Sarah Popp, Sarita Dhounchak, Charmaine Simeonovic

Isolated mouse islets were dispersed into single cells using Accutase (Millipore; 250 µl/500 islets). 4-8 x 104 islet cells were transferred to individual wells of a 96 well culture plate (CELLSTAR, Greiner Bio-one) for immediate staining for flow c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c07a50519f9b7320b20119628375a71d
https://doi.org/10.17504/protocols.io.bmgjk3un

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