Zobrazeno 1 - 3
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pro vyhledávání: '"Sari Liitti"'
Autor:
Adrian Goldman, Sari Liitti, Tuomo Glumoff, Pirkko Heikinheimo, Veli-Pekka Jaakola, Arnab Kapat, Heikki Heimo
Publikováno v:
Bioseparation. 9:167-172
The objective is to generate milligram quantities of recombinant human α2C2 adrenergic receptor for X-ray crystallographic studies. It has been cloned in Saccharomyces cerevisiae, and the production level is at best about 13 pmol/mg of membrane prot
Publikováno v:
Protein expression and purification. 22(1)
Large quantities of correctly folded, pure α 2 -adrenergic receptor protein are needed for structural analysis. We report here the first efficient method to purify human α 2 -adrenergic receptor subtype C2 to homogeneity from recombinant yeast Sacc
Autor:
Anne Marjamäki, Sari Liitti, Markku Jalkanen, Marja-Terttu Matikainen, Jukka Hellman, Jaana Kallio, Heini Närvä
Publikováno v:
Biochemical and biophysical research communications. 233(1)
Monoclonal antibodies (Mabs) against human alpha2C2-adrenergic receptor (alpha2C2-AR) were raised in mice and characterized. Bacterially expressed fusion protein consisting a sequence from the putative third intracellular loop (amino acids 213-343) o