A role for the Gram-negative outer membrane in bacterial shape determination.

Autor: Fivenson EM; Department of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115., Rohs PDA; Department of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115., Vettiger A; Department of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115., Sardis MF; Department of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115., Torres G; Department of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115., Forchoh A; Department of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115., Bernhardt TG; Department of Microbiology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115.; HHMI, Chevy Chase, MD 20815.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2023 Aug 29; Vol. 120 (35), pp. e2301987120. Date of Electronic Publication: 2023 Aug 22.

Preproteins couple the intrinsic dynamics of SecA to its ATPase cycle to translocate via a catch and release mechanism.

Autor: Krishnamurthy S; KU Leuven, University of Leuven, Rega Institute, Department of Microbiology and Immunology, 3000 Leuven, Belgium., Sardis MF; KU Leuven, University of Leuven, Rega Institute, Department of Microbiology and Immunology, 3000 Leuven, Belgium., Eleftheriadis N; KU Leuven, University of Leuven, Rega Institute, Department of Microbiology and Immunology, 3000 Leuven, Belgium., Chatzi KE; KU Leuven, University of Leuven, Rega Institute, Department of Microbiology and Immunology, 3000 Leuven, Belgium., Smit JH; KU Leuven, University of Leuven, Rega Institute, Department of Microbiology and Immunology, 3000 Leuven, Belgium., Karathanou K; Freie Universität Berlin, Department of Physics, Theoretical Molecular Biophysics Group, Arnimallee 14, 14195 Berlin, Germany., Gouridis G; KU Leuven, University of Leuven, Rega Institute, Department of Microbiology and Immunology, 3000 Leuven, Belgium; Molecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, the Netherlands; Structural Biology Division, Institute of Molecular Biology and Biotechnology (IMBB-FORTH), Nikolaou Plastira 100, Heraklion, Crete, Greece., Portaliou AG; KU Leuven, University of Leuven, Rega Institute, Department of Microbiology and Immunology, 3000 Leuven, Belgium., Bondar AN; Freie Universität Berlin, Department of Physics, Theoretical Molecular Biophysics Group, Arnimallee 14, 14195 Berlin, Germany; University of Bucharest, Faculty of Physics, Atomiștilor 405, 077125 Măgurele, Romania; Forschungszentrum Jülich, Institute of Computational Biomedicine, IAS-5/INM-9, Wilhelm-Johnen Straße, 5428 Jülich, Germany., Karamanou S; KU Leuven, University of Leuven, Rega Institute, Department of Microbiology and Immunology, 3000 Leuven, Belgium., Economou A; KU Leuven, University of Leuven, Rega Institute, Department of Microbiology and Immunology, 3000 Leuven, Belgium. Electronic address: tassos.economou@kuleuven.be.

Publikováno v: Cell reports [Cell Rep] 2022 Feb 08; Vol. 38 (6), pp. 110346.

Long-Lived Folding Intermediates Predominate the Targeting-Competent Secretome.

Autor: Tsirigotaki A; KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Molecular Bacteriology, 3000 Leuven, Belgium., Chatzi KE; KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Molecular Bacteriology, 3000 Leuven, Belgium., Koukaki M; Institute of Molecular Biology and Biotechnology, FoRTH, University of Crete, 70013 Heraklion, Crete, Greece., De Geyter J; KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Molecular Bacteriology, 3000 Leuven, Belgium., Portaliou AG; KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Molecular Bacteriology, 3000 Leuven, Belgium., Orfanoudaki G; Institute of Molecular Biology and Biotechnology, FoRTH, University of Crete, 70013 Heraklion, Crete, Greece., Sardis MF; KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Molecular Bacteriology, 3000 Leuven, Belgium., Trelle MB; Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark., Jørgensen TJD; Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark., Karamanou S; KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Molecular Bacteriology, 3000 Leuven, Belgium., Economou A; KU Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Laboratory of Molecular Bacteriology, 3000 Leuven, Belgium. Electronic address: tassos.economou@kuleuven.be.

Publikováno v: Structure (London, England : 1993) [Structure] 2018 May 01; Vol. 26 (5), pp. 695-707.e5. Date of Electronic Publication: 2018 Apr 05.

Preprotein mature domains contain translocase targeting signals that are essential for secretion.

Autor: Chatzi KE; Laboratory of Molecular Bacteriology, Department of Microbiology and Immunology, Rega Institute for Medical Research, Katholieke Universiteit Leuven, 3000 Leuven, Belgium., Sardis MF; Laboratory of Molecular Bacteriology, Department of Microbiology and Immunology, Rega Institute for Medical Research, Katholieke Universiteit Leuven, 3000 Leuven, Belgium., Tsirigotaki A; Laboratory of Molecular Bacteriology, Department of Microbiology and Immunology, Rega Institute for Medical Research, Katholieke Universiteit Leuven, 3000 Leuven, Belgium., Koukaki M; Institute of Molecular Biology and Biotechnology FoRTH, Iraklio, 71110 Crete, Greece., Šoštarić N; Laboratory of Molecular Bacteriology, Department of Microbiology and Immunology, Rega Institute for Medical Research, Katholieke Universiteit Leuven, 3000 Leuven, Belgium., Konijnenberg A; Biomolecular and Analytical Mass Spectrometry Group, Department of Chemistry, University of Antwerp, 2000 Antwerp, Belgium., Sobott F; Biomolecular and Analytical Mass Spectrometry Group, Department of Chemistry, University of Antwerp, 2000 Antwerp, Belgium., Kalodimos CG; Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455., Karamanou S; Laboratory of Molecular Bacteriology, Department of Microbiology and Immunology, Rega Institute for Medical Research, Katholieke Universiteit Leuven, 3000 Leuven, Belgium., Economou A; Laboratory of Molecular Bacteriology, Department of Microbiology and Immunology, Rega Institute for Medical Research, Katholieke Universiteit Leuven, 3000 Leuven, Belgium tassos.economou@kuleuven.be.; Institute of Molecular Biology and Biotechnology FoRTH, Iraklio, 71110 Crete, Greece.

Publikováno v: The Journal of cell biology [J Cell Biol] 2017 May 01; Vol. 216 (5), pp. 1357-1369. Date of Electronic Publication: 2017 Apr 12.

Identification of small-molecule inhibitors against SecA by structure-based virtual ligand screening.

Autor: De Waelheyns E; Laboratory of Molecular Bacteriology, Rega Institute for Medical Research, Department of Microbiology and Immunology, KU Leuven, Leuven, Belgium., Segers K; Laboratory of Molecular Bacteriology, Rega Institute for Medical Research, Department of Microbiology and Immunology, KU Leuven, Leuven, Belgium., Sardis MF; Laboratory of Molecular Bacteriology, Rega Institute for Medical Research, Department of Microbiology and Immunology, KU Leuven, Leuven, Belgium., Anné J; Laboratory of Molecular Bacteriology, Rega Institute for Medical Research, Department of Microbiology and Immunology, KU Leuven, Leuven, Belgium., Nicolaes GA; Cardiovascular Research Institute Maastricht, Department of Biochemistry, Maastricht University, Maastricht, The Netherlands., Economou A; Laboratory of Molecular Bacteriology, Rega Institute for Medical Research, Department of Microbiology and Immunology, KU Leuven, Leuven, Belgium.; Institute of Molecular Biology and Biotechnology-FoRTH, Department of Biology-University of Crete, Iraklio, Crete, Greece.

Publikováno v: The Journal of antibiotics [J Antibiot (Tokyo)] 2015 Nov; Vol. 68 (11), pp. 666-73. Date of Electronic Publication: 2015 May 20.