Zobrazeno 1 - 10
of 27
pro vyhledávání: '"Sarantos Kyriakopoulos"'
Autor:
Wen Qin Tang, Peiqing Zhang, Jong Kwang Hong, Gavin Teo, Dong-Yup Lee, Hsueh Lee Lim, Sarantos Kyriakopoulos, Ying Swan Ho, Yee Jiun Kok, Xuezhi Bi, Meiyappan Lakshmanan, Andy Hee-Meng Tan, Say Kong Ng, Alison P. Lee, Swan Li Poh
Publikováno v:
Biotechnology and Bioengineering. 116:2117-2129
Chinese hamster ovary (CHO) cells are the most prevalent mammalian cell factories for producing recombinant therapeutic proteins due to their ability to synthesize human-like post-translational modifications and ease of maintenance in suspension cult
Publikováno v:
Methods. 102:26-35
In this study, we analyzed multi-omics data and subsets thereof to establish reference codon usage biases for codon optimization in synthetic gene design. Specifically, publicly available genomic, transcriptomic, proteomic and translatomic data for m
Autor:
Kenneth Lee, Alison Mason, Sarantos Kyriakopoulos, Karen M. Polizzi, Cleo Kontoravdi, Christopher A. Sellick, Si Nga Sou
Publikováno v:
Biotechnology and Bioengineering. 112:1165-1176
The application of mild hypothermic conditions to cell culture is a routine industrial practice used to improve recombinant protein production. However, a thorough understanding of the regulation of dynamic cellular processes at lower temperatures is
Publikováno v:
Pharmaceutical Bioprocessing. 2:389-401
As efforts for selecting high-producing cells during cell line development are moving toward the use of high-throughput, automated methods, there is considerable promise for identifying suitable markers arising from transcriptomic, proteomic and meta
Autor:
Rudiyanto Gunawan, Kok Siong Ang, Dong-Yup Lee, Seongkyu Yoon, Meiyappan Lakshmanan, Sarantos Kyriakopoulos, Zhuangrong Huang
Publikováno v:
Biotechnology journal. 13(3)
Kinetic modeling is the most suitable framework to describe the dynamic behavior of mammalian cell culture although its industrial application is still in its infancy. Herein, the authors reviewed mammalian bioprocess relevant kinetic models, and fou
Publikováno v:
Analytical Biochemistry. 443:172-180
Glycosylation is a critical attribute of therapeutic proteins given its impact on the clinical safety and efficacy of these molecules. The biochemical process of glycosylation is inextricably dependent on metabolism and ensuing availability of nucleo
Autor:
Pedro Mendes, Dong-Yup Lee, Michael Hanscho, Nathan E. Lewis, Douglas B. Kell, Meiyappan Lakshmanan, Jahir M. Gutierrez, Alex Thomas, Natalie J. Gardiner, Joanne K. Liu, Shangzhong Li, Paul D. Dobson, Juergen Zanghellini, Hooman Hefzi, Kok Siong Ang, Camila A. Orellana, Veronica Martinez, Neil Swainston, Nicole Borth, Lars K. Nielsen, Daniel C. Zielinski, Kieran Smallbone, Sarantos Kyriakopoulos, Judy Brewer, Lake-Ee Quek
Publikováno v:
Springer US
Metabolomics : Official journal of the Metabolomic Society, vol 12, iss 7
Metabolomics
Swainston, N, Smallbone, K, Hefzi, H, Dobson, P D, Brewer, J, Hanscho, M, Zielinski, D C, Ang, K S, Gardiner, N, Gutierrez, J M, Kyriakopoulos, S, Lakshmanan, M, Li, S, Liu, J K, Martínez, V S, Orellana, C A, Quek, L-E, Thomas, A, Zanghellini, J, Borth, N, Lee, D-Y, Nielsen, L K, Kell, D, Lewis, N E & Mendes, P 2016, ' Recon 2.2 : from reconstruction to model of human metabolism ', Metabolomics : Official journal of the Metabolomic Society, vol. 12, pp. 109 . https://doi.org/10.1007/s11306-016-1051-4
Swainston, N, Smallbone, K, Hefzi, H, Dobson, P D, Brewer, J, Hanscho, M, Zielinski, D C, Ang, K S, Gardiner, N J, Gutierrez, J M, Kyriakopoulos, S, Lakshmanan, M, Li, S, Liu, J K, Martínez, V S, Orellana, C A, Quek, L E, Thomas, A, Zanghellini, J, Borth, N, Lee, D Y, Nielsen, L K, Kell, D B, Lewis, N & Mendes, P 2016, ' Recon 2.2 : from reconstruction to model of human metabolism ', Metabolomics, vol. 12, 109 . https://doi.org/10.1007/s11306-016-1051-4
Metabolomics : Official journal of the Metabolomic Society, vol 12, iss 7
Metabolomics
Swainston, N, Smallbone, K, Hefzi, H, Dobson, P D, Brewer, J, Hanscho, M, Zielinski, D C, Ang, K S, Gardiner, N, Gutierrez, J M, Kyriakopoulos, S, Lakshmanan, M, Li, S, Liu, J K, Martínez, V S, Orellana, C A, Quek, L-E, Thomas, A, Zanghellini, J, Borth, N, Lee, D-Y, Nielsen, L K, Kell, D, Lewis, N E & Mendes, P 2016, ' Recon 2.2 : from reconstruction to model of human metabolism ', Metabolomics : Official journal of the Metabolomic Society, vol. 12, pp. 109 . https://doi.org/10.1007/s11306-016-1051-4
Swainston, N, Smallbone, K, Hefzi, H, Dobson, P D, Brewer, J, Hanscho, M, Zielinski, D C, Ang, K S, Gardiner, N J, Gutierrez, J M, Kyriakopoulos, S, Lakshmanan, M, Li, S, Liu, J K, Martínez, V S, Orellana, C A, Quek, L E, Thomas, A, Zanghellini, J, Borth, N, Lee, D Y, Nielsen, L K, Kell, D B, Lewis, N & Mendes, P 2016, ' Recon 2.2 : from reconstruction to model of human metabolism ', Metabolomics, vol. 12, 109 . https://doi.org/10.1007/s11306-016-1051-4
Introduction The human genome-scale metabolic reconstruction details all known metabolic reactions occurring in humans, and thereby holds substantial promise for studying complex diseases and phenotypes. Capturing the whole human metabolic reconstruc
Autor:
Juergen Zanghellini, Jae Seong Lee, Yingxiang Huang, Daniel C. Zielinski, Daniel Ley, Shangzhong Li, Camila A. Orellana, Philipp Spahn, Bernhard O. Palsson, Nathan E. Lewis, Lasse Ebdrup Pedersen, David E. Ruckerbauer, Sarantos Kyriakopoulos, Natalia E Jiménez, Mikael Rørdam Andersen, Deniz Baycin-Hizal, Veronica Martinez, Kok Siong Ang, Lake-Ee Quek, Zachary A. King, Aarash Bordbar, Harish Nagarajan, Nicole Borth, Helene Faustrup Kildegaard, Anne Mathilde Lund, Dong-Yup Lee, Jahir M. Gutierrez, Alyaa M. Abdel-Haleem, Tune Wulff, Ziomara P. Gerdtzen, Bjørn G. Voldborg, Lars K. Nielsen, Giuseppe Paglia, Alex Thomas, Hooman Hefzi, Meiyappan Lakshmanan, Michael Hanscho, Michael J. Betenbaugh, Johnny Arnsdorf, Nicolás Loira
Publikováno v:
Cell systems, vol 3, iss 5
Cell Syst.
Artículos CONICYT
CONICYT Chile
instacron:CONICYT
Cell Syst.
Artículos CONICYT
CONICYT Chile
instacron:CONICYT
Chinese hamster ovary (CHO) cells dominate biotherapeutic protein production and are widely used in mammalian cell line engineering research. To elucidate metabolic bottlenecks in protein production and to guide cell engineering and bioprocess optimi
Autor:
Paul Christakopoulos, Christina Vafiadi, Peter Biely, Ján Hirsch, Sarantos Kyriakopoulos, Evangelos Topakas
Publikováno v:
FEBS Letters. 584:543-548
Three acetyl esterases (AcEs) from the saprophytic bacteria Cellvibrio japonicus and Clostridium thermocellum, members of the carbohydrate esterase (CE) family 2, were tested for their activity against a series of model substrates including partially
Autor:
Delmas, Maxime1 (AUTHOR) maxime.delmas@idiap.ch, Wysocka, Magdalena2 (AUTHOR) magdalena.wysocka@digitalecmt.org, Freitas, André3 (AUTHOR) andre.freitas@idiap.ch
Publikováno v:
Computational Linguistics. Sep2024, Vol. 50 Issue 3, p953-1000. 48p.