Zobrazeno 1 - 3
of 3
pro vyhledávání: '"Sarah R. Braford"'
Autor:
Jeng-Jong Shieh, Peter O. Olins, Maire Helena Caparon, William F. Hood, Kumnan Paik, Ann M. Donnelly, Bauer Christopher S, John P. McKearn, Sarah R. Braford, Polazzi Joseph O, John W. Thomas, Jon A. Klover, Alan Michael Easton, David L. Zeng, Mark Allen Abrams, Joseph K. Welply, Barbara K. Klein
Publikováno v:
Experimental Hematology. 27:1746-1756
A combinatorial mutagenesis strategy was used to create a collection of nearly 500 variants of human interleukin 3 (IL-3), each with four to nine amino acid substitutions clustered within four linear, nonoverlapping regions of the polypeptide. The va
Autor:
Karl J. Mathis, Robert A. Heeren, Ned R. Siegel, Barbara Kure Klein, Edith Y. Wong, Sarah R. Braford, Bruce F. Bishop, Christine E. Smith, Claire E. Kotts, Ramnath Seetharam, William C. Tacon
Publikováno v:
Gene. 68(2)
The synthesis, processing and secretion of insulin-like growth factor-1 (IGF-1 or somatomedin-C) fused to LamB and OmpF secretion leader sequences in Escherichia coli have been investigated. Expression and secretion of IGF-1 was achieved. The major p
Autor:
Bruce F. Bishop, Serdar Aykent, Robert A. Heeren, Sarah R. Braford, Karl J. Mathis, Claire E. Kotts, Barbara Kure Klein, Michael J. Jennings, Christine E. Smith, Ned R. Siegel, Ramnath Seetharam, Edith Y. Wong
Publikováno v:
Biochemical and biophysical research communications. 155(1)
Summary Partial misincorporation of Lys for Arg has been observed for the Arg residues of IGF-1 when the molecule is expressed in Escherichia coli using a synthetic gene with the low frequency AGA codon encoding all six Arg residues and yeast preferr