Siderophores as tools and treatments

Autor: Á. Tamás Gräff, Sarah M. Barry

Publikováno v: npj Antimicrobials and Resistance, Vol 2, Iss 1, Pp 1-17 (2024)

Abstract In the search for iron, an essential element in many biochemical processes, microorganisms biosynthesise dedicated chelators, known as siderophores, to sequester iron from their environment and actively transport the siderophore complex into

Externí odkaz: https://doaj.org/article/31632142fc4745c8af434d804a5f9fee

Redesigning Enzymes for Biocatalysis: Exploiting Structural Understanding for Improved Selectivity

Autor: Yaoyu Ding, Gustavo Perez-Ortiz, Jessica Peate, Sarah M. Barry

Publikováno v: Frontiers in Molecular Biosciences, Vol 9 (2022)

The discovery of new enzymes, alongside the push to make chemical processes more sustainable, has resulted in increased industrial interest in the use of biocatalytic processes to produce high-value and chiral precursor chemicals. Huge strides in pro

Externí odkaz: https://doaj.org/article/483d7b4b79df4f49bcf30d6e04fc2bdc

Discovery and Rational Mutagenesis of Methionine Sulfoxide Reductase Biocatalysts To Expand the Substrate Scope of the Kinetic Resolution of Chiral Sulfoxides

Autor: Silvia Anselmi, Alexandra T. P. Carvalho, Angela Serrano-Sanchez, Jose L. Ortega-Roldan, Jill Caswell, Iman Omar, Gustavo Perez-Ortiz, Sarah M. Barry, Thomas S. Moody, Daniele Castagnolo

Publikováno v: ACS Catalysis. 13:4742-4751

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2ca5f955617460597bc0d1098c7b90e9
https://doi.org/10.1021/acscatal.3c00372

A mild and chemoselective CALB biocatalysed synthesis of sulfoxides exploiting the dual role of AcOEt as solvent and reagent

Autor: Thomas S. Moody, Silvia Anselmi, Daniele Castagnolo, Siyu Liu, Sarah M. Barry, Seong Heun Kim

Publikováno v: Organic & Biomolecular Chemistry. 19:156-161

A mild, chemoselective and sustainable biocatalysed synthesis of sulfoxides has been developed exploiting CALB and using AcOEt with a dual role of more environmentally friendly reaction solvent and enzyme substrate. A series of sulfoxides, including

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3d58479d7a209c198b75ab32ebdd063
https://doi.org/10.1039/d0ob01966f

Catalytic Mechanism of Aromatic Nitration by Cytochrome P450 TxtE: Involvement of a Ferric-Peroxynitrite Intermediate

Autor: Lona M. Alkhalaf, Andrew W. Munro, Sarah M. Barry, Hafiz Saqib Ali, M. Qadri E. Mubarak, Sam P. de Visser, Nigel S. Scrutton, Savvas Louka, Gregory L. Challis, Derren J. Heyes

Publikováno v: Journal of the American Chemical Society
Louka, S, Barry, S M, Heyes, D J, Mubarak, M Q E, Ali, H S, Alkhalaf, L M, Munro, A W, Scrutton, N S, Challis, G L & de Visser, S P 2020, ' Catalytic Mechanism of Aromatic Nitration by Cytochrome P450 TxtE : Involvement of a Ferric-Peroxynitrite Intermediate ', Journal of the American Chemical Society, vol. 142, no. 37, pp. 15764-15779 . https://doi.org/10.1021/jacs.0c05070

The cytochromes P450 are heme-dependent enzymes that catalyze many vital reaction processes in the human body related to biodegradation and biosynthesis. They typically act as mono-oxygenases; however, the recently discovered P450 subfamily TxtE util

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91471ce75d84594a01ac13a32ff7bd75
https://pubmed.ncbi.nlm.nih.gov/32811149