Zobrazeno 1 - 10
of 80
pro vyhledávání: '"Sarah L. Westcott"'
Autor:
Kelly L. Sovacool, Sarah L. Westcott, M. Brodie Mumphrey, Gabrielle A. Dotson, Patrick D. Schloss
Publikováno v:
mSphere, Vol 7, Iss 1 (2022)
ABSTRACT Assigning amplicon sequences to operational taxonomic units (OTUs) is an important step in characterizing microbial communities across large data sets. A notable difference between de novo clustering and database-dependent reference clusteri
Externí odkaz:
https://doaj.org/article/fa947efa4bb64b7babf1f49793d984cc
Autor:
Sarah L. Westcott, Patrick D. Schloss
Publikováno v:
mSphere, Vol 2, Iss 2 (2017)
ABSTRACT Assignment of 16S rRNA gene sequences to operational taxonomic units (OTUs) is a computational bottleneck in the process of analyzing microbial communities. Although this has been an active area of research, it has been difficult to overcome
Externí odkaz:
https://doaj.org/article/4e82c537decc40d890eac4e2fbeafad9
Autor:
Patrick D. Schloss, Sarah L. Westcott, Kelly Sovacool, M. Brodie Mumphrey, Gabrielle A. Dotson
Publikováno v:
mSphere. 7
Assigning amplicon sequences to operational taxonomic units (OTUs) is often an important step in characterizing the composition of microbial communities across large datasets. OptiClust, a de novo OTU clustering method, has been shown to produce high
Autor:
Patrick D. Schloss, Matthew L. Jenior, Charles C. Koumpouras, Sarah L. Westcott, Sarah K. Highlander
Publikováno v:
PeerJ, Vol 4, p e1869 (2016)
Over the past 10 years, microbial ecologists have largely abandoned sequencing 16S rRNA genes by the Sanger sequencing method and have instead adopted highly parallelized sequencing platforms. These new platforms, such as 454 and Illumina’s MiSeq,
Externí odkaz:
https://doaj.org/article/43cb332f045c4333963edc4f949dfc0c
Autor:
Sarah L. Westcott, Patrick D. Schloss
Publikováno v:
PeerJ, Vol 3, p e1487 (2015)
Background. 16S rRNA gene sequences are routinely assigned to operational taxonomic units (OTUs) that are then used to analyze complex microbial communities. A number of methods have been employed to carry out the assignment of 16S rRNA gene sequence
Externí odkaz:
https://doaj.org/article/18ddd2e128d44bc5a6e2f6c922b45fd0
Publikováno v:
PLoS ONE, Vol 6, Iss 12, p e27310 (2011)
The advent of next generation sequencing has coincided with a growth in interest in using these approaches to better understand the role of the structure and function of the microbial communities in human, animal, and environmental health. Yet, use o
Externí odkaz:
https://doaj.org/article/2e8475189fad4adcb6cda8300c9b90c7
OptiClust: Improved method for assigning amplicon-based sequence data to operational taxonomic units
Autor:
Sarah L. Westcott, Patrick D. Schloss
Assignment of 16S rRNA gene sequences to operational taxonomic units (OTUs) is a computational bottleneck in the process of analyzing microbial communities. Although this has been an active area of research, it has been difficult to overcome the time
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8383bd435a9abbbf2b01f1e4a94be11
https://doi.org/10.1101/096537
https://doi.org/10.1101/096537
Publikováno v:
Applied and Environmental Microbiology. 79:5112-5120
Rapid advances in sequencing technology have changed the experimental landscape of microbial ecology. In the last 10 years, the field has moved from sequencing hundreds of 16S rRNA gene fragments per study using clone libraries to the sequencing of m
Autor:
Patrick D. Schloss, Sarah L. Westcott
Publikováno v:
PeerJ
PeerJ, Vol 3, p e1487 (2015)
PeerJ, Vol 3, p e1487 (2015)
Background.16S rRNA gene sequences are routinely assigned to operational taxonomic units (OTUs) that are then used to analyze complex microbial communities. A number of methods have been employed to carry out the assignment of 16S rRNA gene sequences
Autor:
Sarah L Westcott, Patrick Schloss
Background. 16S rRNA gene sequences are routinely assigned to operational taxonomic units (OTUs) that are then used to analyze complex microbial communities. A number of methods have been employed to carry out the assignment of 16S rRNA gene sequence
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2dda4c3e158eebebb27a20234a06e0f1
https://doi.org/10.7287/peerj.preprints.1511v1
https://doi.org/10.7287/peerj.preprints.1511v1