The SWI/SNF chromatin remodeling complex influences transcription by RNA polymerase I in Saccharomyces cerevisiae.

Autor: Yinfeng Zhang, Susan J Anderson, Sarah L French, Martha L Sikes, Olga V Viktorovskaya, Jacalyn Huband, Katherine Holcomb, John L Hartman, Ann L Beyer, David A Schneider

Publikováno v: PLoS ONE, Vol 8, Iss 2, p e56793 (2013)

SWI/SNF is a chromatin remodeling complex that affects transcription initiation and elongation by RNA polymerase II. Here we report that SWI/SNF also plays a role in transcription by RNA polymerase I (Pol I) in Saccharomyces cerevisiae. Deletion of t

Externí odkaz: https://doaj.org/article/a9bd2f5d4e3943e29182cd609e4cdcd5

Nascent Transcript Folding Plays a Major Role in Determining RNA Polymerase Elongation Rates

Autor: Benjamin D. Goddard, Aleksandra Helwak, Elisabeth Petfalski, Tomasz W. Turowski, David Tollervey, Sarah L. French

Publikováno v: Molecular Cell
Turowski, T, Petfalski, E, Goddard, B D, French, S L, Helwak, A & Tollervey, D 2020, ' Nascent transcript folding plays a major role in determining RNA polymerase elongation rates ', Molecular Cell, vol. 79, no. 3, pp. 488-503.e11 . https://doi.org/10.1016/j.molcel.2020.06.002

Summary Transcription elongation rates influence RNA processing, but sequence-specific regulation is poorly understood. We addressed this in vivo, analyzing RNAPI in S. cerevisiae. Mapping RNAPI by Miller chromatin spreads or UV crosslinking revealed

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a4ebe0de430ec8a2e5befdc70a6e39e
https://doi.org/10.1016/j.molcel.2020.06.002

Cohesion promotes nucleolar structure and function

Autor: Brian D. Slaughter, Rhonda Trimble Ross, Ann L. Beyer, Bethany Harris, Tania Bose, Sarah L. French, Jay R. Unruh, Fei Wang, Ying Zhang, Jennifer L. Gerton, Shuai Lu, Kenneth K. Lee

Publikováno v: Molecular Biology of the Cell

Mutations in the cohesin acetyltransferase Eco1 or the cohesin ring compromise nucleolar function in budding yeast. A mutation in Eco1 that is associated with the human disease Roberts syndrome compromises looping interactions at the ribosomal DNA an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8456d76756880756b8ee2a0aea42fdaa
https://doi.org/10.1091/mbc.e13-07-0377

Distinguishing the Roles of Topoisomerases I and II in Relief of Transcription-Induced Torsional Stress in Yeast rRNA Genes

Autor: Robert D. Hontz, Aziz El Hage, David Tollervey, Jeffrey S. Smith, M. Mitchell Smith, Tashima E. Lambert, Ann L. Beyer, Sarah L. French, Martha L. Sikes, Yvonne N. Osheim

Publikováno v: French, S L, Sikes, M L, Hontz, R D, Osheim, Y N, Lambert, T E, El Hage, A, Smith, M M, Tollervey, D, Smith, J S & Beyer, A L 2011, ' Distinguishing the Roles of Topoisomerases I and II in Relief of Transcription-Induced Torsional Stress in Yeast rRNA Genes ', Molecular and Cellular Biology, vol. 31, no. 3, pp. 482-494 . https://doi.org/10.1128/MCB.00589-10

To better understand the role of topoisomerase activity in relieving transcription-induced supercoiling, yeast genes encoding rRNA were visualized in cells deficient for either or both of the two major topoisomerases. In the absence of both topoisome

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b4e1371892ccafbdddf262b184008dc
https://doi.org/10.1128/mcb.00589-10

Role of Histone Deacetylase Rpd3 in Regulating rRNA Gene Transcription and Nucleolar Structure in Yeast

Autor: Manabu Sato, Melanie Oakes, Loan Vu, Imran Siddiqi, John P. Aris, Sarah L. French, Masayasu Nomura, Ann L. Beyer

Publikováno v: Molecular and Cellular Biology. 26:3889-3901

The 35S rRNA genes at the RDN1 locus in Saccharomyces cerevisiae can be transcribed by RNA polymerase (Pol) II in addition to Pol I, but Pol II transcription is usually silenced. The deletion of RRN9 encoding an essential subunit of the Pol I transcr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2726260912c72331bee6946a7a2c3b91
https://doi.org/10.1128/mcb.26.10.3889-3901.2006